Inserm Bio-Santé Toulouse

IBST

Inserm Bio-Santé Toulouse (IBST) is composed by two Inserm platforms located in two Research Institutes of Toulouse (I2MC -Metabolic and Cardiovascular Research Institute- and CRCT -Cancer Research Institute-) for a total of four engineers. Since 2017, these platforms assist users from the design of the single-cell experiments to the data analysis, including single-cell libraries generation and sequencing. The platform propose all the solutions and protocols commercialized by 10x Genomics, with strong expertise in single-cell gene expression, CITE-seq, Immune profiling (TCR, BCR) and spatial transcriptomics.

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TEAM

Expertises

EQUIPMENTS

CONTACT

Team

Engineer - GeT-Sante platform
Emeline LHUILLIER

Engineer - GeT-Sante platform
Frédéric MARTINS

Engineer - GeT-Sante platform

Emeline LHUILLIER

Engineer in GeT-Santé Core Facility

Emeline Lhuillier received her Master degree in Molecular Biology option Genetics and Biodiversity Management, from the Pierre & Marie Curie University, Paris, France, in 2005. After several experiences in conservation biology and population genetics labs, she joined the Genome and Transcriptome Core Facility (GeT, Toulouse, France) in May 2011 as a Next Generation Sequencing Engineer in INRAE. Still working as an engineer on GeT, she was recruted in the French National Institute of Health and Medical research in 2014 on an other site of the core facility, and finally joined the I2MC lab in September 2018.

Since January 2020, Emeline Lhuillier is co-manager of the GeT-Santé platform with Frédéric Martins, and offers her expertise to research teams from the health community for advising and conducting their RNA-seq (or broader NGS) projects.

Main domains of expertise: Next Generation Sequencing, RNA-sequencing, Genomics, Transcriptomics

Email:emeline.lhuillier@inserm.fr

Phone:05 61 32 56 49

Engineer - GeT-Sante platform

Frédéric MARTINS

Engineer on the GeT-Sante platform

Career path
Frédéric Martins obtained his Licence Professionnelle in Biotechnologies, specialising in Techniques and Applications in Cellular and Molecular Biology, from the Université Victor Segalen, Bordeaux, France, in 2003. After several experiences in the field of human genetics and antibody production, he joined the Genome & Transcriptome Platform (GeT, Toulouse, France) in January 2008, as an assistant-engineer in QPCR and HD genotyping at INRAe. Still as an assistant-engineer at GeT, he was recruited at Inserm in 2012 on another site of the platform at I2MC. In 2018, he passed the competitive examination for engineer.

Since January 2020, Frédéric Martins is co-responsible for the GeT-Santé platform with Emeline Lhuillier, and offers his expertise to research teams of the Health community to advise them and carry out their Single Cell Omics projects.

Field of expertise
Single Cell Omics, QPCR, ddPCR, Transcriptomics.

Email:frederic.martins@inserm.fr

Phone:05 61 35 56 49

Engineer

Carine VALLE

Email:carine.valle@inserm.fr

Engineer

Emeline SAROT

Thesis Tittle : Régulation du rétrovirus Gypsy par le locus Flamenco de Drosophila melanogaster : mise en évidence d’un mécanisme d’inhibition par homologie de séquence

Year : 2005

University : Université Montpellier II

Email::emeline.sarot@inserm.fr

EXPERTISES

SINGLE CELL

Reveal the full complexity of cellular diversity, cell by cell

“Chromium Single Cell Gene Expression” provides single cell transcriptome 3′ gene expression and multiomic capabilities to profile tens of thousands of cells. This technology allows to explore the full complexity of cellular diversity, cellular heterogeneity, novel targets, and biomarkers with combined gene expression and surface protein expression in each cell

SPATIAL OMIC

“Visium Spatial Gene Expression” is a next-generation molecular profiling solution for classifying tissue based on total mRNA. This technology allows to map the whole transcriptome with morphological context in FFPE or fresh-frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

Séquençage NGS

RNAseq, ChIPseq, WGS…: support in project design, library construction, coordination with sequencing and bioinfo analysis platforms.

EQUIPMENTS

2 Chromium Single Cell Controllers

The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. Powered by Next GEM technology, the Chromium Controller enables integrated analysis of single cells at massive scale.

NextSeq 550

The NovaSeq 6000 System unleashes a new era in sequencing with groundbreaking innovations, providing users with the throughput, speed, and flexibility to complete projects faster and more economically than ever before. Leveraging proven Illumina next-generation sequencing (NGS) technology, multiple flow cell types, two library loading workflows, and various read length combinations, the NovaSeq 6000 System enables effective throughput scaling to suit virtually any study needs.

NovaSeq6000

The NovaSeq 6000 System unleashes a new era in sequencing with groundbreaking innovations, providing users with the throughput, speed, and flexibility to complete projects faster and more economically than ever before. Leveraging proven Illumina next-generation sequencing (NGS) technology, multiple flow cell types, two library loading workflows, and various read length combinations, the NovaSeq 6000 System enables effective throughput scaling to suit virtually any study needs.

CONTACT

Do not hesitate to contact us for your projects.

liste.ibst@inserm.fr

Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4

Tél. : 05 61 32 56 00

Contactez-nous

Horaires

Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45

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