Histology

 

The platform offers histology services on paraffin-embedded or frozen tissue: sections, staining, support and advice for all your projects.

 

TEAM

Expertises

EQUIPMENTS

BOOKING/CONTACT

TEAM

 
Tous / AllPlateform leaderTechnical staffScientific adviser
Plateform leader

Christèle SEGURA

MCU-PH

Céline GUILBEAU-FRUGIER

Plateform leader

Christèle SEGURA

Engineer in Histology Core Facility

After studying at the University of Sciences and Techniques of Languedoc in Montpellier, she obtained a DEA “Adaptations and survival in extreme environments” issued by the Jean Monnet University of Saint-Etienne. Technician in the “Neurobiology of Rhythmic and Seasonal Functions” laboratory at the University Louis Pasteur in Strasbourg then in the “Histology-Embryology” laboratory of the Faculty of Medicine of the University of Paul Sabatier in Toulouse, she deepens her knowledge in histology. After 17 years spent on the Vectorology platform of the CRCT (INSERM / Université Toulouse 3) where she acquired solid skills in the production of viral vectors, cloning and cell culture, she joined the I2MC to take responsibility for the histology platform and bring its skills and know-how to the scientific community.

Areas of expertise: Histology, Immunohistochemistry, In Situ hybridization, cell culture, vectorology

MCU-PH

Céline GUILBEAU-FRUGIER

Medical Doctor, Physician-scientists doctor

Career path
Céline is a pathologist, a forensic medical doctor and the director of an electron microscopic platform. She obtained her PhD degree in pharmacology in 2012 at the university of Toulouse. She joined our team in 2013 wherein she developed the use of electron microscopy to explore the cardiac tissue.

Main domain of expertise
Myocardial morphology, electron microscopy, histology

SCIENTIFIC COMMITTEE

 

Céline GUILBEAU-FRUGIER   Barbara GARMY-SUSINI
Coralie FONTAINE   Marie MARQUES
Alexia ZAKAROFF-GIRARD   Alicia VAUCLARD

EXPERTISES

INCLUSION

 

Inclusion of your samples in paraffin blocks.

 

 

SECTION

 

Production of your tissue sections from frozen or paraffin embedded samples.

COLORATION

 

The platform performs routine or special staining on frozen or paraffin tissue sections for you.

 

TRAINING, EXPERTISE

 Practical training in the use of the equipment…

– Advice on sample preparation…

– Assistance in setting up your projects…

EQUIPMENTS

Cryostats

– 2 cryostats Thermo NX50 are available on the platform
Thermo NX50

Microtomes

– 1 microtome equipped with a Niagara transfer system
MM France UM340E

– 1 microtome Leica RM 2245

Automate

– 1 automatic staining machine
Autostainer XL/Leica

Inclusion

– 1 automatic dehydration machine
STP-121/MMFrance

– 1 station for inclusion
LEICA EG1150C / EG1150H

Cytospin

– 1 cytospin 4 Thermo Scientific

BOOKING & CONTACT

 

Booking cryostats and microtome
(after prior training)

Ordering services on a contract basis
(for holders of an @inserm.fr address)

COST

INTERNAL RULES

CUSTOMER FOR REQUEST

A request ?

recent publications

eIF4A inhibition circumvents uncontrolled DNA replication mediated by 4E-BP1 loss in pancreatic cancer. David Müller, Sauyeun Shin, Théo Goullet de Rugy, Rémi Samain, Romain Baer, Manon Strehaiano, Laia Masvidal-Sanz, Julie Guillermet-Guibert, Christine Jean, Yoshinori Tsukumo, Nahum Sonenberg, Frédéric Marion, Nicolas Guilbaud, Jean-Sébastien Hoffmann, Ola Larsson, Corinne Bousquet, Stéphane Pyronnet, Yvan Martineau. JCI Insight. 2019. Pubmed

NEWS

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère. Creating endometriosis organoids from patients’ lesions to test drug candidates. This is the challenge of the EndoTreat project led by a team from Toulouse.

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logotype i2mc

Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4

Tél. : 05 61 32 56 00

Contactez-nous

 

Horaires

Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45