Phi

 

The Phi platform offers a wide range of techniques from sample preparation to imaging and analysis to visualise and quantify biological processes at different scales, from molecules to organs.

TEAM

Expertises

BOOKING/CONTACT

TEAM

 
Tous / AllPlateform leaderTechnical staffScientific adviser
Plateform leader

Rémy FLORES-FLORES

Plateform leader

Christèle SEGURA

Principal scientist

Damien RAMEL

MCU-PH

Céline GUILBEAU-FRUGIER

Plateform leader

Rémy FLORES-FLORES

IE (INSERM Research Engineer) 

Since January 2019, he is the head of the I2MC imaging department where he brings his expertise and skills in optical microscopy and image analysis to the scientific community.

Career path
Rémy Flores-Flores obtained a master’s degree in Physics, specialising in the Exploration of the Living Environment in 2012 from the Joseph Fourrier University and a master’s degree in Optical and Laser Instrumentations in 2013. In 2014 he joined the imaging platform and the team of P.F. Lenne at IBDM (Institut de Biologie du Développement de Marseille) where he deepened his knowledge in instrumentation and optical microscopy for biology and image analysis. In 2018 he was recruited as an engineer at INSERM and joined the TRI imaging platform at I2MC.

Field of expertise 
Photonic microscopy,

 

Plateform leader

Christèle SEGURA

Engineer in Histology Core Facility

After studying at the University of Sciences and Techniques of Languedoc in Montpellier, she obtained a DEA “Adaptations and survival in extreme environments” issued by the Jean Monnet University of Saint-Etienne. Technician in the “Neurobiology of Rhythmic and Seasonal Functions” laboratory at the University Louis Pasteur in Strasbourg then in the “Histology-Embryology” laboratory of the Faculty of Medicine of the University of Paul Sabatier in Toulouse, she deepens her knowledge in histology. After 17 years spent on the Vectorology platform of the CRCT (INSERM / Université Toulouse 3) where she acquired solid skills in the production of viral vectors, cloning and cell culture, she joined the I2MC to take responsibility for the histology platform and bring its skills and know-how to the scientific community.

Areas of expertise: Histology, Immunohistochemistry, In Situ hybridization, cell culture, vectorology

Principal scientist

Damien RAMEL

CNRS researcher

Cellular and molecular biologist. His research focuses on the study of molecular determinants of vascular smooth muscle cells biology in normal and physiopathological conditions.

Career path
Master’s degree and PhD in cancerology at Paul Sabatier university, Toulouse (2004-2009), Post-doc experience at University of Montréal (Canada, 2010-2013). Permanent position at CNRS (CBI, Toulouse) in 2013. Joined Laffargue’s team in 2017.

Main domains of expertise
Cell signaling, Endocytosis, cell migration, cytoskeleton, small GTPases, phosphoinositides, cellular imaging (FRET, Optogenetics, spectral imaging).

MCU-PH

Céline GUILBEAU-FRUGIER

Medical Doctor, Physician-scientists doctor

Career path
Céline is a pathologist, a forensic medical doctor and the director of an electron microscopic platform. She obtained her PhD degree in pharmacology in 2012 at the university of Toulouse. She joined our team in 2013 wherein she developed the use of electron microscopy to explore the cardiac tissue.

Main domain of expertise
Myocardial morphology, electron microscopy, histology

EXPERTISES

Samples preparation

– Paraffin Inclusions
– Sample sections
– Staining
– Advice and training

PHOTONIC MICROSCOPY

Expertise in multiple acquisition modalities (transmitted light, fluorescence, spectral) at multiple scales (molecule, organelle, cell, tissue) from sample preparation to analysis.

PROCESSING AND ANALYSIS

Advice and implementation in image processing (visualisation, segmentation and measurement extraction) and data processing (transformation, analysis and visualisation)

ADAPTIVE MICROSCOPY

Development of adaptive microscopy experiments, combining image analysis and microscope automation.

BOOKING & CONTACT

 

Reservation of histology equipments

Reservation of Imaging equipments

reservation of analyzes stations 

COSTS

Internal rules

CUSTOMER FOR REQUEST

A request ?

Partners

ImaBIO

ImaBIO

publications 

Effects of novel Btk and Syk inhibitors on platelet functions alone and in combination in vitro and in vivo, J Series J, Ribes A, Garcia C, Souleyreau P, Bauters A, Morschhauser F, Jürgensmeier JM, Sié P, Ysebaert L, Payrastre B. Thromb Haemost. 2020. Pubmed

Tamoxifen Accelerates Endothelial Healing by Targeting ERα in Smooth Muscle Cells. Zahreddine R, Davezac M, Smirnova N, Buscato M, Lhuillier E, Lupieri A, Solinhac R, Vinel A, Vessieres E, Henrion D, Renault MA, Gadeau AP, Flouriot G, Lenfant F, Laffargue M, Métivier R, Arnal JF, Fontaine C. Circ Res. 2020. Pubmed

Smooth muscle cells-derived CXCL10 prevents endothelial healing through PI3Kγ-dependent T cells response. Lupieri A, Smirnova NF, Solinhac R, Malet N, Benamar M, Saoudi A, Santos-Zas I, Zeboudj L, Ait-Oufella H, Hirsch E, Ohayon P, Lhermusier T, Carrié D, Arnal JF, Ramel D, Gayral S, Laffargue M.. Cardiovasc Res. 2020. Pubmed

Mitochondrial 4-HNE derived from MAO-A promotes mitoCa 2+ overload in chronic postischemic cardiac remodeling. Santin Y, Fazal L, Sainte-Marie Y, Sicard P, Maggiorani D, Tortosa F, Yücel YY, Teyssedre L, Rouquette J, Marcellin M, Vindis C, Shih JC, Lairez O, Burlet-Schiltz O, Parini A, Lezoualc’h F, Mialet-Perez J. Cell Death Differ. 2020 Pubmed

In vitro and in vivo cardioprotective and metabolic efficacy of vitamin E TPGS/Apelin. Leme Goto P, Cinato M, Merachli F, Vons B, Jimenez T, Marsal D, Todua N, Loi H, Santin Y, Cassel S, Blanzat M, Tronchere H, Dejugnat C, Kunduzova O, Boal F. J Mol Cell Cardiol. 2020Pubmed

Actualités

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère

EndoTreat: research project conducted in partnership with the Toulouse University Hospital (E. Chantalat), INSERM-I2MC (F. Lenfant) and the start-up company Urosphère. Creating endometriosis organoids from patients’ lesions to test drug candidates. This is the challenge of the EndoTreat project led by a team from Toulouse.

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Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4

Tél. : 05 61 32 56 00

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Horaires

Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45