team A. Bouloumié
DINAMIX: Adipose tissues and vasculo-metabolic flexibility
Our research aims to characterize the molecular and cellular mechanisms involved in metabolic adaptation in the context of natural and accelerated obesity-induced aging with a focus on the characterization of cellular and functional heterogeneity of white, beige and brown adipose depots in vascular-metabolic flexibility.
L’Équipe
Anne BOULOUMIE-DIEHL
Xavier COLLET
Jean GALITSKY
Anaïs BRIOT
Florence TATIN
Nathalie BOULET
Alex AUPETIT
Emmanuelle MARCHAUD
Léopold Devinaux
Pauline DECAUNES BESSEDE
Valentin Saunier
Laura Zapata-Medrano
Metabolic function of microvascular endothelium: Heterogeneity, nutrient sensing and handling
Principal investigators : Anaïs Briot, Anne Bouloumié
Our work on native and primary microvascular endothelial cells from subcutaneous and visceral human and mouse adipose depots aims at 1) identifying the cellular and molecular mechanisms involved in nutrient sensing and transport, 2) defining the inter- and intra-fat depot phenotypic and functional heterogeneity of endothelial cells and, 3) assessing the impact of obesity and aging on adipose depots endothelial cells metabolic function in human and mice.
From progenitor cells to mature adipocytes: Heterogeneity, differentiation and function
Principal investigators : Jean Galitzky, Anne Bouloumié
Our objectives are to define the intrinsic and extrinsic molecular mechanisms governing the heterogeneity of adipocytes and progenitor cells in terms of their fat (white, beige and brown) and myofibrogenic fate and their intra- and inter-depot niche in physiological and pathological contexts of natural or obesity accelerated aging.
Dynamics and adaptation of adipose depot:
VASCULOMetabolic flexibility and fasting
Principal investigator : Anaïs Briot
Using distinct fasting protocols, our objectives are 1) to identify dynamic combinations of markers (metabolomic, transcriptomic and metagenomic) of metabolic inflexibility, 2) to assess the beneficial and deleterious consequences on the phenotype and functionality of adipose depot cells, and 3) to identify cellular and molecular mechanisms that selectively mimic the beneficial effects of intermittent fasting according to sex, age and metabolic health.
Regulation of postprandial triglyceridemia
Coordinator : Xavier Collet
Postprandial hypertriglyceridemia (PPHT) is a recognized vascular risk factor. The original model of SR-B1-invalidated mice in the intestine (Cre-Villin, iSR-BI KO) will be studied in order to characterize the role of cholesterol-dependent SR-BI in the control of TGPP in relation to FXR and bile acids and the involvement of this metabolic pathway in the development of atherosclerosis under conditions of lipid-enriched diet.
Adaptability of lymphatic endothelium in metabolic diseases
Principal investigators : Florence Tatin, Anais briot, Anne Bouloumie
Florence Tatin is interested in understanding the adaptability and heterogeneity of lymphatic endothelium in metabolic diseases. We are interested to determine the spatial organization of the lymphatic network in adipose tissue microenvironment with new approaches of 3D imaging by light sheet microscopy (IMACTIV-3D). In addition, obesity is well-recognized as an important rick factor for lymphatic dysfunction. We aim to better understand how adipose tissue may influence and interact with lymphatic endothelial cells, and inversely how dysfunction of lymphatic vessels may alter cell-responses of the adipose tissue. Towards this aim, we develop single-cell RNAseq analysis of endothelial cells, and cell sorting strategy associated with cell biology and biochemistry approaches.
RECENT PUBLICATIONS
Obesity of mice lacking VAP-1/SSAO by Aoc3 gene deletion is reproduced in mice expressing a mutated vascular adhesion protein-1 (VAP-1) devoid of amine oxidase activity, Jargaud V, Bour S, Tercé F, Collet X, Valet P, Bouloumié A, Guillemot JC, Mauriège P, Jalkanen S, Stolen C, Salmi M, Smith DJ, Carpéné C. J Physiol Biochem. 2021. Pubmed
Detection of representative variables in complex systems wtih interpretable rules using Core-Clusters. Champion, C., A. C. Brunet, R. Burcelin, J. M. loubes and l. Risser. Algorithms 2021. Pubmed
Molecular phenomics and metagenomics of hepatic steatosis in non-diabetic obese women. Hoyles, L., J. M. Fernandez-Real, M. Federici, M. Serino, J. Abbott, J. Charpentier, C. Heymes, J. L. Luque, E. Anthony, R. H. Barton, J. Chilloux, A. Myridakis, L. Martinez-Gili, J. M. Moreno-Navarrete, F. Benhamed, V. Azalbert, V. Blasco-Baque, J. Puig, G. Xifra, W. Ricart, C. Tomlinson, M. Woodbridge, M. Cardellini, F. Davato, I. Cardolini, O. Porzio, P. Gentileschi, F. Lopez, F. Foufelle, S. A. Butcher, E. Holmes, J. K. Nicholson, C. Postic, R. Burcelin and M. E. Dumas. Nat Med. 2018. Pubmed
Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells. Estève D, Boulet N, Belles C, Zakaroff-Girard A, Decaunes P, Briot A, Veeranagouda Y, Didier M, Remaury A, Guillemot JC, Ledoux S, Dani C, Bouloumié A, Galitzky J. Nat Commun. 2019. Pubmed
Senescence Alters PPARγ (Peroxisome Proliferator-Activated Receptor Gamma)-Dependent Fatty Acid Handling in Human Adipose Tissue Microvascular Endothelial Cells and Favors Inflammation. Briot A, Decaunes P, Volat F, Belles C, Coupaye M, Ledoux S, Bouloumié A. Arterioscler Thromb Vasc Biol. 2018. Pubmed
THEY ARE SUPPORTING OUR RESEARCH
Slider A BOULOUMIE
SFD
FEDER
INSERM
EASD
AFERO
Clarins
Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires
1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4
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