team A. Bouloumié
DINAMIX: Adipose tissues and vasculo-metabolic flexibility
Our research aims to characterize the molecular and cellular mechanisms involved in metabolic adaptation in the context of natural and accelerated obesity-induced aging with a focus on the characterization of cellular and functional heterogeneity of white, beige and brown adipose depots in vascular-metabolic flexibility.
L’Équipe

Anne BOULOUMIE-DIEHL

Xavier COLLET

Anaïs BRIOT

Florence TATIN

Léopold Devineaux

Pauline DECAUNES BESSEDE

Juline MARJOLLET

Anne Gosset
Metabolic function of microvascular endothelium: Heterogeneity, nutrient sensing and handling

Principal investigators : Anaïs Briot, Anne Bouloumié
Our work on native and primary microvascular endothelial cells from subcutaneous and visceral human and mouse adipose depots aims at 1) identifying the cellular and molecular mechanisms involved in nutrient sensing and transport, 2) defining the inter- and intra-fat depot phenotypic and functional heterogeneity of endothelial cells and, 3) assessing the impact of obesity and aging on adipose depots endothelial cells metabolic function in human and mice.
From progenitor cells to mature adipocytes: Heterogeneity, differentiation and function

Principal investigators : Jean Galitzky, Anne Bouloumié
Our objectives are to define the intrinsic and extrinsic molecular mechanisms governing the heterogeneity of adipocytes and progenitor cells in terms of their fat (white, beige and brown) and myofibrogenic fate and their intra- and inter-depot niche in physiological and pathological contexts of natural or obesity accelerated aging.
Dynamics and adaptation of adipose depot:
VASCULOMetabolic flexibility and fasting

Principal investigator : Anaïs Briot
Using distinct fasting protocols, our objectives are 1) to identify dynamic combinations of markers (metabolomic, transcriptomic and metagenomic) of metabolic inflexibility, 2) to assess the beneficial and deleterious consequences on the phenotype and functionality of adipose depot cells, and 3) to identify cellular and molecular mechanisms that selectively mimic the beneficial effects of intermittent fasting according to sex, age and metabolic health.
Regulation of postprandial triglyceridemia
Coordinator : Xavier Collet
Postprandial hypertriglyceridemia (PPHT) is a recognized vascular risk factor. The original model of SR-B1-invalidated mice in the intestine (Cre-Villin, iSR-BI KO) will be studied in order to characterize the role of cholesterol-dependent SR-BI in the control of TGPP in relation to FXR and bile acids and the involvement of this metabolic pathway in the development of atherosclerosis under conditions of lipid-enriched diet.
Adaptability of lymphatic endothelium in metabolic diseases

Principal investigators : Florence Tatin, Anais briot, Anne Bouloumie
Florence Tatin is interested in understanding the adaptability and heterogeneity of lymphatic endothelium in metabolic diseases. We are interested to determine the spatial organization of the lymphatic network in adipose tissue microenvironment with new approaches of 3D imaging by light sheet microscopy (IMACTIV-3D). In addition, obesity is well-recognized as an important rick factor for lymphatic dysfunction. We aim to better understand how adipose tissue may influence and interact with lymphatic endothelial cells, and inversely how dysfunction of lymphatic vessels may alter cell-responses of the adipose tissue. Towards this aim, we develop single-cell RNAseq analysis of endothelial cells, and cell sorting strategy associated with cell biology and biochemistry approaches.
RECENT PUBLICATIONS
Endothelial DLL4 is an Adipose Depot-Specific fasting sensor regulating fatty acid fluxes. Aupetit A, Decaunes P, Belles C, Riant E, Galitzky J, Chapouly C, Laisné M, Flores-Flores R, Chaput B, Vié K, Arnal JF, Bouloumié A, Briot A. Arterioscler Thromb Vasc Biol. 2023 PMID: 36924232. Pubmed
Notch activation shifts the fate decision of senescent progenitors toward myofibrogenesis in human adipose tissue. Boulet N, Briot A, Jargaud V, Estève D, Rémaury A, Belles C, Viana P, Fontaine J, Murphy L, Déon C, Guillemot M, Pech C, Veeranagouda Y, Didier M, Decaunes P, Mouisel E, Carpéné C, Iacovoni JS, Zakaroff-Girard A, Grolleau JL, Galitzky J, Ledoux S, Guillemot JC, Bouloumié A. Aging Cell. 2023 Jan 8:e13776. Pubmed
Subcutaneous Stromal Cells and Visceral Adipocyte Size Are Determinants of Metabolic Flexibility in Obesity and in Response to Weight Loss Surgery. Ledoux S, Boulet N, Belles C, Zakaroff-Girard A, Bernard A, Germain A, Decaunes P, Briot A, Galitzky J, Bouloumié A. Cells. 2022 Nov 9;11(22):3540. Pubmed
The Sexual Dimorphism of Human Adipose Depots. Biomedicines. 2022 Oct 18;10(10):2615. Pubmed
Periprostatic Adipose Tissue Displays a Chronic Hypoxic State that Limits Its Expandability. Am J Pathol. 2022 Jun;192(6):926-942. Pubmed
The Impact of Long-term Physical Inactivity on Adipose Tissue Immunometabolism. J Clin Endocrinol Metab. 2022 Jan 1;107(1):177-191. Pubmed
Expression of the Adipocyte Progenitor Markers MSCA1 and CD36 is Associated With Adipose Tissue Function in Children. Pubmed


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