The platform offers a wide range of imaging and analysis techniques to visualize and quantify biological processes at different scales, from the molecule to the organ.
IE (INSERM Research Engineer)
Since January 2019, he is the head of the I2MC imaging department where he brings his expertise and skills in optical microscopy and image analysis to the scientific community.
Rémy Flores-Flores obtained a master’s degree in Physics, specialising in the Exploration of the Living Environment in 2012 from the Joseph Fourrier University and a master’s degree in Optical and Laser Instrumentations in 2013. In 2014 he joined the imaging platform and the team of P.F. Lenne at IBDM (Institut de Biologie du Développement de Marseille) where he deepened his knowledge in instrumentation and optical microscopy for biology and image analysis. In 2018 he was recruited as an engineer at INSERM and joined the TRI imaging platform at I2MC.
Field of expertise
Cellular and molecular biologist. His research focuses on the study of molecular determinants of vascular smooth muscle cells biology in normal and physiopathological conditions.
Master’s degree and PhD in cancerology at Paul Sabatier university, Toulouse (2004-2009), Post-doc experience at University of Montréal (Canada, 2010-2013). Permanent position at CNRS (CBI, Toulouse) in 2013. Joined Laffargue’s team in 2017.
Main domains of expertise
Cell signaling, Endocytosis, cell migration, cytoskeleton, small GTPases, phosphoinositides, cellular imaging (FRET, Optogenetics, spectral imaging).
– Theoretical and practical training
– Advice for the realisation of your projects
– Acquisition and analysis of your images
– Collaboration and development
Expertise in multiple acquisition modalities (transmitted light, fluorescence, spectral) at multiple scales (molecule, organelle, cell, tissue) from sample preparation to analysis.
PROCESSING AND ANALYSIS
Advice and implementation in image processing (visualisation, segmentation and measurement extraction) and data processing (transformation, analysis and visualisation)
Development of adaptive microscopy experiments, combining image analysis and microscope automation.
Confocal microscope LSM780
Confocal laser scanning microscope (405, 458, 488, 514, 561 and 633 nm) with spectral detector and cell incubation system.
Confocal microscope LSM900
Confocal laser scanning microscope (405, 488, 561 and 640 nm) with Airyscan 2 Multiplex detector and Focus Map function for acquisition on tissue section.
Cell-Observer Video Microscope
Video microscope with LED illumination (transmitted light and fluorescence), cell incubation system and 2 monochrome cameras (CCD and EM-CCD).
Microscope Elira PS.1
Microscope for: high resolution structured illumination (SIM-3D), single molecule localization nanoscopy (SMLM) and total reflection optical sectioning (TIRF).
Computers dedicated to image and data processing and analysis using the following software and languages: ImageJ/Fiji, Ilastik, QuPath, Imaris, Python.
BOOKING & CONTACT
Reservation of equipment
CUSTOMER FOR REQUEST
A REQUEST ?
PIKfyve-dependent phosphoinositide dynamics in...
The Interplay of Endothelial P2Y Receptors in...
23 et 24 Juin , CBI Toulouse Optogenetics is a...
Effects of novel Btk and Syk inhibitors on platelet functions alone and in combination in vitro and in vivo, J Series J, Ribes A, Garcia C, Souleyreau P, Bauters A, Morschhauser F, Jürgensmeier JM, Sié P, Ysebaert L, Payrastre B. Thromb Haemost. 2020. Pubmed
Tamoxifen Accelerates Endothelial Healing by Targeting ERα in Smooth Muscle Cells. Zahreddine R, Davezac M, Smirnova N, Buscato M, Lhuillier E, Lupieri A, Solinhac R, Vinel A, Vessieres E, Henrion D, Renault MA, Gadeau AP, Flouriot G, Lenfant F, Laffargue M, Métivier R, Arnal JF, Fontaine C. Circ Res. 2020. Pubmed
Smooth muscle cells-derived CXCL10 prevents endothelial healing through PI3Kγ-dependent T cells response. Lupieri A, Smirnova NF, Solinhac R, Malet N, Benamar M, Saoudi A, Santos-Zas I, Zeboudj L, Ait-Oufella H, Hirsch E, Ohayon P, Lhermusier T, Carrié D, Arnal JF, Ramel D, Gayral S, Laffargue M.. Cardiovasc Res. 2020. Pubmed
Mitochondrial 4-HNE derived from MAO-A promotes mitoCa 2+ overload in chronic postischemic cardiac remodeling. Santin Y, Fazal L, Sainte-Marie Y, Sicard P, Maggiorani D, Tortosa F, Yücel YY, Teyssedre L, Rouquette J, Marcellin M, Vindis C, Shih JC, Lairez O, Burlet-Schiltz O, Parini A, Lezoualc’h F, Mialet-Perez J. Cell Death Differ. 2020 Pubmed
In vitro and in vivo cardioprotective and metabolic efficacy of vitamin E TPGS/Apelin. Leme Goto P, Cinato M, Merachli F, Vons B, Jimenez T, Marsal D, Todua N, Loi H, Santin Y, Cassel S, Blanzat M, Tronchere H, Dejugnat C, Kunduzova O, Boal F. J Mol Cell Cardiol. 2020. Pubmed
Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires
1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4
Tél. : 05 61 32 56 00
Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45