Imaging

 

The platform offers a wide range of imaging and analysis techniques to visualize and quantify biological processes at different scales, from the molecule to the organ.

TEAM

Expertises

EQUIPMENTS

BOOKING/CONTACT

Team

 
Tous / AllPlateform leaderTechnical staffScientific adviser
Plateform leader

Rémy FLORES-FLORES

PhD, Engineer

Romina D’ANGELO

Principal scientist

Damien RAMEL

Plateform leader

Rémy FLORES-FLORES

IE (INSERM Research Engineer) 

Since January 2019, he is the head of the I2MC imaging department where he brings his expertise and skills in optical microscopy and image analysis to the scientific community.

Career path
Rémy Flores-Flores obtained a master’s degree in Physics, specialising in the Exploration of the Living Environment in 2012 from the Joseph Fourrier University and a master’s degree in Optical and Laser Instrumentations in 2013. In 2014 he joined the imaging platform and the team of P.F. Lenne at IBDM (Institut de Biologie du Développement de Marseille) where he deepened his knowledge in instrumentation and optical microscopy for biology and image analysis. In 2018 he was recruited as an engineer at INSERM and joined the TRI imaging platform at I2MC.

Field of expertise 
Photonic microscopy,

 

PhD, Engineer

Romina D’ANGELO

Principal scientist

Damien RAMEL

CNRS researcher

Cellular and molecular biologist. His research focuses on the study of molecular determinants of vascular smooth muscle cells biology in normal and physiopathological conditions.

Career path
Master’s degree and PhD in cancerology at Paul Sabatier university, Toulouse (2004-2009), Post-doc experience at University of Montréal (Canada, 2010-2013). Permanent position at CNRS (CBI, Toulouse) in 2013. Joined Laffargue’s team in 2017.

Main domains of expertise
Cell signaling, Endocytosis, cell migration, cytoskeleton, small GTPases, phosphoinositides, cellular imaging (FRET, Optogenetics, spectral imaging).

SCIENTIFIC COMMITTEE

 

Damien RAMEL   Email me 
Frédéric BOAL   Email me 
Virgie BOURLIER   Email me 
Melissa BUSCATO   Email me
Pascal CLERC   Email me 
Jean GALITZKY   Email me 
Anne MAZARS  Email me
Sonia SEVERIN  Email me

EXPERTISES

SERVICES PROVIDED

– Theoretical and practical training
– Advice for the realisation of your projects
– Acquisition and analysis of your images
– Collaboration and development

PHOTONIC MICROSCOPY

Expertise in multiple acquisition modalities (transmitted light, fluorescence, spectral) at multiple scales (molecule, organelle, cell, tissue) from sample preparation to analysis.

PROCESSING AND ANALYSIS

Advice and implementation in image processing (visualisation, segmentation and measurement extraction) and data processing (transformation, analysis and visualisation)

ADAPTIVE MICROSCOPY

Development of adaptive microscopy experiments, combining image analysis and microscope automation.

EQUIPMENTS

Confocal microscope LSM780

Confocal laser scanning microscope (405, 458, 488, 514, 561 and 633 nm) with spectral detector and cell incubation system.

Confocal microscope LSM900

Confocal laser scanning microscope (405, 488, 561 and 640 nm) with Airyscan 2 Multiplex detector and Focus Map function for acquisition on tissue section.

Cell-Observer Video Microscope

Video microscope with LED illumination (transmitted light and fluorescence), cell incubation system and 2 monochrome cameras (CCD and EM-CCD).

Microscope Elira PS.1

Microscope for: high resolution structured illumination (SIM-3D), single molecule localization nanoscopy (SMLM) and total reflection optical sectioning (TIRF).

Analysis stations

Computers dedicated to image and data processing and analysis using the following software and languages: ImageJ/Fiji, Ilastik, QuPath, Imaris, Python.

BOOKING & CONTACT

 

Reservation of equipment

COSTS

INTERNAL RULES

CUSTOMER FOR REQUEST

A REQUEST ?

Actualités

publications

Effects of novel Btk and Syk inhibitors on platelet functions alone and in combination in vitro and in vivo, J Series J, Ribes A, Garcia C, Souleyreau P, Bauters A, Morschhauser F, Jürgensmeier JM, Sié P, Ysebaert L, Payrastre B. Thromb Haemost. 2020. Pubmed

Tamoxifen Accelerates Endothelial Healing by Targeting ERα in Smooth Muscle Cells. Zahreddine R, Davezac M, Smirnova N, Buscato M, Lhuillier E, Lupieri A, Solinhac R, Vinel A, Vessieres E, Henrion D, Renault MA, Gadeau AP, Flouriot G, Lenfant F, Laffargue M, Métivier R, Arnal JF, Fontaine C. Circ Res. 2020. Pubmed

Smooth muscle cells-derived CXCL10 prevents endothelial healing through PI3Kγ-dependent T cells response. Lupieri A, Smirnova NF, Solinhac R, Malet N, Benamar M, Saoudi A, Santos-Zas I, Zeboudj L, Ait-Oufella H, Hirsch E, Ohayon P, Lhermusier T, Carrié D, Arnal JF, Ramel D, Gayral S, Laffargue M.. Cardiovasc Res. 2020. Pubmed

Mitochondrial 4-HNE derived from MAO-A promotes mitoCa 2+ overload in chronic postischemic cardiac remodeling. Santin Y, Fazal L, Sainte-Marie Y, Sicard P, Maggiorani D, Tortosa F, Yücel YY, Teyssedre L, Rouquette J, Marcellin M, Vindis C, Shih JC, Lairez O, Burlet-Schiltz O, Parini A, Lezoualc’h F, Mialet-Perez J. Cell Death Differ. 2020 Pubmed

In vitro and in vivo cardioprotective and metabolic efficacy of vitamin E TPGS/Apelin. Leme Goto P, Cinato M, Merachli F, Vons B, Jimenez T, Marsal D, Todua N, Loi H, Santin Y, Cassel S, Blanzat M, Tronchere H, Dejugnat C, Kunduzova O, Boal F. J Mol Cell Cardiol. 2020Pubmed

PARTNERS

ImaBIO

ImaBIO

logotype i2mc
logotype i2mc

Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4

Tél. : 05 61 32 56 00

Contactez-nous

 

Horaires

Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45