The vocation of this platform is to provide you with the latest innovative technologies and technical support in functional biochemistry.
IE (INSERM Research Engineer)
Alexandre is the founder and manager of the We-Met platform. He is a specialist in protein biochemistry. He manages the platform and is responsible for various training courses and developments.
He started in 2005 at Inserm in Chatenay-Malabry in cardiac signalling and then joined the I2MC in 2010. He then founded the We-Met platform in 2015.
Field of expertise
Protein Biochemistry, Simple Western, Seahorse, ,Biomarkers discovery
Phone:05 61 32 56 09 / 06 82 46 74 78
TCE (Technicienne de recherche de classe exceptionnelle INSERM)
Corinne is expert in protein biochemistry. She took part on several oxydative cell signalisation projects. Now, she helps platform users how to manage their project with protein or biomarker issues.
DUT : technological degree in biological engineering. Corinne was recruted by INSERM in 2009 after a university job in Toulouse. She is at the functional biochemistry facility We-Met since 2019.
Protein biochemistry. Simple western technology. Biomarker research.
IR (Research Engineer CDD INSERM)
Steven participates in the various projects of the We-met platform, he is notably involved in the development of new detection techniques and biochemical assays.
After a PhD from Paul Sabatier University and a post-doctoral experience at the Institute of Digestive Health Research in Toulouse, he will join the We-Met platform at the Institute of Metabolic and Cardiovascular Diseases (I2MC) in 2021.
Field of expertise
Biochemistry, cell biology and in vivo model studies
Senior staff scientist
After undergraduate studies in biology and biochemistry in Sweden (Linköping) and USA (Boulder, CO), and PhD studies in Lund (Sweden) and in Toulouse, Henrik Laurell, defended his thesis in 1998 at the Lund University. After 2 post-doctoral positions in Toulouse, he obtained an Inserm position in 2004 as a staff scientist. His interest in methodology in molecular biology (and especially in qPCR), resulted in the development of the ValidPrime method and more recently QSeeD, a qPCR-based method to determine cell death. Since 2019 he has been using proximity-based biotinylation (TurboID) to define the proxisome/interactome of proteins of interest. In 2021 he joined the We-Met plateau to help the teams in the institute to develop projects based on the TurboID technology.
Main domains of expertise:
Molecular biology, qPCR, gene expression, molecular cloning, protein biochemistry, protein-protein interactions, proximity-based biotinylation
PhD, Director of research, Group leader
Laurent Martinez received his PhD degree in physiopathology from Paul Sabatier University (Toulouse, France). He is Director of research at Inserm and head a research team at I2MC since 2011.
After his PhD, Laurent Martinez did a post-doc at Columbia University (NYC, USA) in the laboratory of Pr. Alan Tall, working on the role of ABC transporters in lipoprotein metabolism. He did a second post-doc at the Mitochondrial Biology Unit in Cambridge (former Dunn Human Nutrition Unit, UK) in the team of Sir John Walker. He then integrated the CPTP research Institute in Toulouse, heading first an Inserm Avenir Team then his team moved to I2MC in 2011.
Main area of expertise
Lipoproteins, mitochondria, purinergic receptor, biomarkers, metabolic and cardiovascular disorders.
Tél/phone:+33 (0)5 31 22 41 47
The platform is equipped with 1 Wes and 2 Jess.
The simple western is an automated capillary western technology. Accessible in autonomy after training or in service provision.
The Seahorse analyser provides a metabolic profile by measuring micro variations in O² consumption and extracellular acidification of a cell culture, tissue or mitochondria.
ELLA enables biomarker assays to be performed using a microfluidic array.
Wide range of human analytes, in development in rodents, open cartridges for R&D.
Multi-mode microplate reader. Wavelength filters to read Absorbance, Fluorescence (UV and visible), TRF or Luminometry (2 injectors).
Milo allows single cell western analysis using a slide containing thousands of units.
In each unit, a living cell is lysed and the proteins separated by electrophoresis.
Teams can come and read their classic western blots (chemiluminescence, colorimetric and fluorescence) and stain-free gels using 2 high sensitivity readers (Chemidoc Touch and Chemidoc MP).
BOOKING & CONTACT
Booking of equipments
Tarifs du plateau
A Request ?
Identification of inflammatory subgroups of schizophrenia and bipolar disorder patients with HERV-W ENV antigenemia by unsupervised cluster analysis. Tamouza R, Meyer U, Foiselle M, Richard JR, Lu CL, Boukouaci W, Le Corvoisier P, Barrau C, Lucas A, Perron H, Leboyer M. Transl Psychiatry. 2021 Jul 6;11(1):377. doi: 10.1038/s41398-021-01499-0. Erratum in: Transl Psychiatry. 2021 Sep 1;11(1):447. PMID: 34230451; PMCID: PMC8260666. Pubmed
Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Laudette M, Sainte-Marie Y, Cousin G, Bergonnier D, Belhabib I, Brun S, Formoso K, Laib L, Tortosa F, Bergoglio C, Marcheix B, Borén J, Lairez O, Fauconnier J, Lucas A, Mialet-Perez J, Moro C, Lezoualc’h F. Cell Death Dis. 2021 Sep. Pubmed
Mechanisms of artemether toxicity on single cardiomyocytes and protective effect of nanoencapsulation. Moreira Souza AC, Grabe-Guimarães A, Cruz JDS, Santos-Miranda A, Farah C, Teixeira Oliveira L, Lucas A, Aimond F, Sicard P, Mosqueira VCF, Richard S. Br J Pharmacol. 2020. Pubmed
Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and mice. Abot A, Wemelle E, Laurens C, Paquot A, Pomie N, Carper D, Bessac A, Mas Orea X, Fremez C, Fontanie M, Lucas A, Lesage J, Everard A, Meunier E, Dietrich G, Muccioli GG, Moro C, Cani PD, Knauf C. Gut. 2020. Pubmed
Galanin enhances systemic glucose metabolism through enteric Nitric Oxide Synthase-expressed neurons. Abot A, Lucas A, Bautzova T, Bessac A, Fournel A, Le-Gonidec S, Valet P, Moro C, Cani PD, Knauf C. Mol Metab. 2018. Pubmed
Multifunctionnal Mitochondrial Epac1 Controls Myocardial Cell Death. Fazal L, Laudette M, Paula-Gomes S, Pons S, Conte C, Tortosa F, Sicard P, Sainte-Marie Y, Biseerier M, Lairez O, Lucas A, Roy J, Ghaleh B, Fauconnier J, Mialet-Perez J, Lezoualc’h F. Circ Res 2017. Pubmed
On the 7th of October at 11h00, George CAPUTA(*) will speak by videoconference to present Nature’s editorial policy, in particular in the field of metabolic and cardiovascular diseases, and to answer your questions.
The We-Met platform and the I2MC are hosting the 1st Simple Western French Users’ Day on 11 and 12 October. Free, but mandatory, registration for all Simple Western users.
The MetaToul-Lipidomics platform has developed a method for profiling plasma lipids by supercritical fluid chromatography (SFC) coupled with mass spectrometry.
Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires
1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4
Tél. : 05 61 32 56 00
Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45