FONCTIONAL 

BIOCHIMISTRY (WE-MET)

 

The vocation of this platform is to provide you with the latest innovative technologies and technical support in functional biochemistry.

 

TEAM

Expertises

EQUIPMENTS

BOOKING/CONTACT

TEAM

 
Tous / AllTechnical staffPlateform leader
PhD, Engineer INSERM Plateform Leader

Alexandre LUCAS

TCE We-Met

Corinne BERNIS

PhD, Engineer

Steven FRIED

Researcher INSERM

Henrik LAURELL

Team leader

Laurent MARTINEZ

PhD, Engineer INSERM Plateform Leader

Alexandre LUCAS

IE (INSERM Research Engineer)

Alexandre is the founder and manager of the We-Met platform. He is a specialist in protein biochemistry. He manages the platform and is responsible for various training courses and developments.

Career path
He started in 2005 at Inserm in Chatenay-Malabry in cardiac signalling and then joined the I2MC in 2010. He then founded the We-Met platform in 2015.

Field of expertise
Protein Biochemistry, Simple Western, Seahorse, ,Biomarkers discovery

Email:alexandre.lucas@inserm.fr

Phone:05 61 32 56 09 / 06 82 46 74 78

TCE We-Met

Corinne BERNIS

TCE (Technicienne de recherche de classe exceptionnelle INSERM) 

Corinne is expert in protein biochemistry. She took part on several oxydative cell signalisation projects. Now, she helps platform users how to manage their project with protein or biomarker issues.

Career path
DUT : technological degree in biological engineering. Corinne was recruted by INSERM in 2009 after a university job in Toulouse. She is at the functional biochemistry facility We-Met since 2019.

Expertise area
Protein biochemistry. Simple western technology. Biomarker research.

PhD, Engineer

Steven FRIED

IR (Research Engineer CDD INSERM)

Steven participates in the various projects of the We-met platform, he is notably involved in the development of new detection techniques and biochemical assays.

Career path
After a PhD from Paul Sabatier University and a post-doctoral experience at the Institute of Digestive Health Research in Toulouse, he will join the We-Met platform at the Institute of Metabolic and Cardiovascular Diseases (I2MC) in 2021.

Field of expertise 
Biochemistry, cell biology and in vivo model studies

Researcher INSERM

Henrik LAURELL

Senior staff scientist

After undergraduate studies in biology and biochemistry in Sweden (Linköping) and USA (Boulder, CO), and PhD studies in Lund (Sweden) and in Toulouse, Henrik Laurell, defended his thesis in 1998 at the Lund University. After 2 post-doctoral positions in Toulouse, he obtained an Inserm position in 2004 as a staff scientist. His interest in methodology in molecular biology (and especially in qPCR), resulted in the development of the ValidPrime method and more recently QSeeD, a qPCR-based method to determine cell death. Since 2019 he has been using proximity-based biotinylation (TurboID) to define the proxisome/interactome of proteins of interest. In 2021 he joined the We-Met plateau to help the teams in the institute to develop projects based on the TurboID technology.

Main domains of expertise:
Molecular biology, qPCR, gene expression, molecular cloning, protein biochemistry, protein-protein interactions, proximity-based biotinylation

Team leader

Laurent MARTINEZ

PhD, Director of research, Group leader

Laurent Martinez received his PhD degree in physiopathology from Paul Sabatier University (Toulouse, France). He is Director of research at Inserm and head a research team at I2MC since 2011.

Career path
After his PhD, Laurent Martinez did a post-doc at Columbia University (NYC, USA) in the laboratory of Pr. Alan Tall, working on the role of ABC transporters in lipoprotein metabolism. He did a second post-doc at the Mitochondrial Biology Unit in Cambridge (former Dunn Human Nutrition Unit, UK) in the team of Sir John Walker. He then integrated the CPTP research Institute in Toulouse, heading first an Inserm Avenir Team then his team moved to I2MC in 2011.

Main area of expertise
Lipoproteins, mitochondria, purinergic receptor, biomarkers, metabolic and cardiovascular disorders.

Email:laurent.martinez@inserm.fr

Tél/phone:+33 (0)5 31 22 41 47

SCIENTIFIC COMMITTEE

 

Melissa BUSCATO   Email me 
Julie COURNET   Email me 
Florian DAVID   Email me 
Thibaut DUPARC   Email me
Victorine DOUIN   Email me 
Aline MAIRAL   Email me 
Nicole MALET   Email me 
Yannis Sainte-Marie   Email me
Julien VIAUD   Email me

EXPERTISES

SIMPLE WESTERN

BIOMARKERS

METABOLISM

TRAINING

EQUIPMENTS

Simple Western

The platform is equipped with 1 Wes and 2 Jess.

The simple western is an automated capillary western technology. Accessible in autonomy after training or in service provision.

Seahorse

The Seahorse analyser provides a metabolic profile by measuring micro variations in O² consumption and extracellular acidification of a cell culture, tissue or mitochondria.

ELLA

ELLA enables biomarker assays to be performed using a microfluidic array.

Wide range of human analytes, in development in rodents, open cartridges for R&D.

Tecan

Multi-mode microplate reader. Wavelength filters to read Absorbance, Fluorescence (UV and visible), TRF or Luminometry (2 injectors).

Milo

Milo allows single cell western analysis using a slide containing thousands of units.

In each unit, a living cell is lysed and the proteins separated by electrophoresis.

Chemidoc

Teams can come and read their classic western blots (chemiluminescence, colorimetric and fluorescence) and stain-free gels using 2 high sensitivity readers (Chemidoc Touch and Chemidoc MP).

BOOKING & CONTACT

 

Booking of equipments

Tarifs du plateau

Règlement intérieur

Fiche Projet

A Request ?

RECENT publications 

Partners

Protein Simple

Protein Simple

Geneuro

Geneuro

Agilent

Agilent

RECENT publications

Identification of inflammatory subgroups of schizophrenia and bipolar disorder patients with HERV-W ENV antigenemia by unsupervised cluster analysis. Tamouza R, Meyer U, Foiselle M, Richard JR, Lu CL, Boukouaci W, Le Corvoisier P, Barrau C, Lucas A, Perron H, Leboyer M. Transl Psychiatry. 2021 Jul 6;11(1):377. doi: 10.1038/s41398-021-01499-0. Erratum in: Transl Psychiatry. 2021 Sep 1;11(1):447. PMID: 34230451; PMCID: PMC8260666. Pubmed

Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Laudette M, Sainte-Marie Y, Cousin G, Bergonnier D, Belhabib I, Brun S, Formoso K, Laib L, Tortosa F, Bergoglio C, Marcheix B, Borén J, Lairez O, Fauconnier J, Lucas A, Mialet-Perez J, Moro C, Lezoualc’h F.  Cell Death Dis. 2021 Sep. Pubmed

Mechanisms of artemether toxicity on single cardiomyocytes and protective effect of nanoencapsulation. Moreira Souza AC, Grabe-Guimarães A, Cruz JDS, Santos-Miranda A, Farah C, Teixeira Oliveira L, Lucas A, Aimond F, Sicard P, Mosqueira VCF, Richard S. Br J Pharmacol. 2020. Pubmed

Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and mice. Abot A, Wemelle E, Laurens C, Paquot A, Pomie N, Carper D, Bessac A, Mas Orea X, Fremez C, Fontanie M, Lucas A, Lesage J, Everard A, Meunier E, Dietrich G, Muccioli GG, Moro C, Cani PD, Knauf C. Gut. 2020. Pubmed

Galanin enhances systemic glucose metabolism through enteric Nitric Oxide Synthase-expressed neurons. Abot A, Lucas A, Bautzova T, Bessac A, Fournel A, Le-Gonidec S, Valet P, Moro C, Cani PD, Knauf C. Mol Metab. 2018. Pubmed

Multifunctionnal Mitochondrial Epac1 Controls Myocardial Cell Death. Fazal L, Laudette M, Paula-Gomes S, Pons S, Conte C, Tortosa F, Sicard P, Sainte-Marie Y, Biseerier M, Lairez O, Lucas A, Roy J, Ghaleh B, Fauconnier J, Mialet-Perez J, Lezoualc’h F. Circ Res 2017Pubmed

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Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

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