Cytometry
& Cell SORTING

 

The platform offers state-of-the-art cytometers (analyzers/sorters), and the necessary expertise to use them. Open to all, we will support you in the development of your projects.

 

TEAM

Expertises

EQUIPMENTS

BOOKING/CONTACT

TEAM

 
Tous / AllPlateform leaderTechnical staff
PHD, Operational manager of Cytometry Facility, Engineer

Alexia ZAKAROFF-GIRARD

PhD, Ingineer INSERM

Elodie RIANT

Team leader

Anne BOULOUMIE-DIEHL

PHD, Operational manager of Cytometry Facility, Engineer

Alexia ZAKAROFF-GIRARD

PhD, Operational manager, Engineer

Alexia Zakaroff-Girard received her PhD from Claude Bernard University (Lyon) in 1997 where she studied the role of biologically active phospholipids in immune response. She completed post-doctoral training at I2MC in adipose tissue physiology. Since 10 years, she is heading the Cytometry and Cell Sorting Facility at I2MC, offering a wealth of experience and resources in the use of flow cytometry for the analyses and sorting of cell populations harvested from solid tissues for academic and private research investigators.

Main domains of expertise:
Flow cytometry, Cell sorting, Adipose tissue physiology, Cellular Biology

PhD, Ingineer INSERM

Elodie RIANT

PhD, Engineer INSERM

Elodie Riant received her PhD from Paul Sabatier University (Toulouse) in 2009 where she studied the protective role of oestrogens on obesity and idiabetes type 2. She completed post-doctoral training at I2MC in adipose tissue physiology. Since 2016, she is working on the Cytometry and Cell Sorting Facility at I2MC, offering a wealth of experience and resources in the use of flow cytometry for the analyses and sorting of cell populations harvested from solid tissues for academic and private research investigators.

Main domains of expertise:
Flow cytometry, Cell sorting, Adipose tissue physiology, Cellular Biology

Team leader

Anne BOULOUMIE-DIEHL

PhD, HDR, INSERM Research Director, Team Leader

Career path
PhD in pharmacology, director M. Lafontan, Université P Sabatier, Toulouse (1994); Post-doc, Cardiovascular Institute (R. Busse), Frankfurt, Germany (1994-1998); CRCN INSERM U598 (1998-2001); Kovalevskaja award, Frankfurt, Germany (2002-2006); AVENIR award, I2MR, Toulouse (2007-2011), Since 2012, INSERM team leader, I2MC.

Main domains of expertise  
Adipose tissue, progenitor, immune cells, endothelial cells, adipogenesis, inflammation, fibrosis, senescence, aging, obesity.

SCIENTIFIC COMMITTEE

 

Anne BOULOUMIE   Email me 
Nathalie PIZZINAT   Email me 
Cédric GARCIA   Email me 
Claire LAURENS   Email me
Yannis Sainte-Marie   Email me 
Florence TATIN   Email me 
Françoise LENFANT   Email me 
Audrey CASEMAYOU   Email me 
Véronique GIGOUX   Email me 
Frédéric MARTINS   Email me 

EXPERTISES

TRAINING, ADVICE, EXPERTISE

– Theoretical and practical training on analysers
– Help with panel design
– Advice on the implementation of your projects
– Help with the analysis of your results
– Data processing (FlowJo)

REALIZATION / EXPERTISE

On request, we can carry out your experiments from acquisition to data analysis.
And we perform cell sorting on all types of samples.

RESEARCH & DEVELOPMENT

We can assist you in the development of new flow cytometry approaches to advance your projects.

APPLICATIONS

 

Numerous analysis applications are possible: membrane and intracellular multi-labelling, cell cycle, apoptosis, multiplex analysis… even cell sorting!

EQUIPMENTS

BD LSRII Fortessa

 Analyzer 4 lasers
405, 488, 561, 633 nm
16 parameters

Access by reservation for users with mandatory training.

Assistance available by appointment.

BD FACS Verse

Analyzer 1 laser
488 nm
6 parameters

Free access by reservation for users with mandatory training.

Technical assistance is available by appointment.

Influx BD Cell Sorter

High speed sorter 4 lasers
405, 488, 561, 633nm

Possibility to sort 6 populations simultaneously, cloning unit.

Sorting is carried out by the staff as a service by appointment.

Analyses Stations

We have 2 analysis stations with free access to rework your data on DIVA 8, FlowJo.

Assistance available by appointment.

BOOKING & CONTACT

 

Booking slots for the Fortessa and Verse analysers via this schedule.

Reservation of a sorting slot by tray staff by request only.

COSTS

INTERNAL RULES

CUSTOMER FOR REQUEST

A REQUEST ?

NEWs

RECENT publications

Selective Cardiomyocyte Oxidative Stress Leads to Bystander Senescence of Cardiac Stromal Cells. Martini H, Lefevre L, Sayir S, Itier R, Maggiorani D, Dutaur M, Marsal DJ, Roncalli J, Pizzinat N, Cussac D, Parini A, Mialet-Perez J, Douin-Echinard V. Int J Mol Sci. 2021 . Pubmed

Circulating CD14high CD16low intermediate blood monocytes as a biomarker of ascites immune status and ovarian cancer progression. Prat M, Le Naour A, Coulson K, Lemée F, Leray H, Jacquemin G, Rahabi MC, Lemaitre L, Authier H, Ferron G, Barret JM, Martinez A, Ayyoub M, Delord JP, Gladieff L, Tabah-Fisch I, Prost JF, Couderc B, Coste A. J Immunother Cancer. 2020. Pubmed

Targeting Myeloperoxidase Disrupts Mitochondrial redox balance and overcomes cytarabine resistance in human acute myeloid leukemia. Hosseini M, Rezvani H, Aroua N, Bosc C, Farge T, Saland E, Guyonnet-Dupérat V, Zaghdoudi S, Jarrou L, Larrue C, Sabatier M, Mouchel PL, Gotanègre M, Piechaczyk M, Bossis G, Récher C, Sarry JE. Cancer Res. 2019. Pubmed

Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells. Estève D, Boulet N, Belles C, Zakaroff-Girard A, Decaunes P, Briot A, Veeranagouda Y, Didier M, Remaury A, Guillemot JC, Ledoux S, Dani C, Bouloumié A, Galitzky J. Nat Commun. 2019. Pubmed

Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes. Krystle Veerman, Claire Tardiveau, Frédéric Martins, Juliette Coudert,
Jean-Philippe Girard. Cell Reports. 2019
. Pubmed

Partners

logotype i2mc
logotype i2mc

Inserm/UPS UMR 1297 - I2MC Institut des Maladies Métaboliques et Cardiovasculaires

1 avenue Jean Poulhès - BP 84225 - 31432 Toulouse Cedex 4

Tél. : 05 61 32 56 00

Contactez-nous

 

Horaires

Du lundi au vendredi
8h30 - 12h30 / 13h45 -16h45