Team B. Payrastre

Lipidomic and signaling in platelet production, thrombosis and cell homeostasis (LIPSIPLAT)

Our laboratory studies phosphoinositides (PI) metabolism in health and disease. We use integrative and translational approaches to decipher PI-signalling and functions in various models including megakaryocyte, platelet and skeletal muscle. We also develop original translational research in the field of cardiovascular diseases in close interaction with clinicians.

TEAM MEMBERS

 
Tous / AllTeam leaderResearchersCliniciansStudentsTechnical staff
Team leader

Bernard PAYRASTRE

Principal scientist

Marie-Pierre GRATACAP

Principal scientist

Karim HNIA

Principal scientist

Sonia SEVERIN

Principal scientist

Julien VIAUD

Chercheure

Marie VANDROMME

Clinician

Christophe COGNARD

Clinician

Thibault LHERMUSIER

Clinician

Agnès RIBES

Clinician

Pierre SIÉ

Clinician

Sophie VOISIN

Clinician, PhD student

Jean DARCOURT

Clinician, PhD student

Fanny VARDON-BOUNES

Research engineer INSERM

Gaëtan CHICANNE

Engineer

Cédric GARCIA

Engineer

Jean-Marie Xuereb

Engineer, Inserm

Anne MAZARS

Engineer

Marine MARSEILLAC

PhD student

Laura BOUVET

PhD student

Mathilde VAUCOURT

PhD student

Maxime BORRET

PhD student

Oportune KPOTOR

PhD student

Nesrine HIFDI

Team leader

Bernard PAYRASTRE

Professor of Haematology/Haemostasis (PU-PH), Medical Faculty Toulouse Purpan.

Responsible of the Functional Unit of “Cardiovascular Haemostasis”, Laboratory of Haematology, University Hospital of Toulouse.

Short description of positions held or career path
Diplomas: HDR, PhD thesis, Medical Pedagogy, Specialized electron microscopy.
2001-08: Director of Research at CNRS, group leader at Inserm U563, Toulouse.
2008: Professor of Haematology/Haemostasis (PU-PH), Medical Faculty Toulouse Purpan.

Teaching
Pathophysiology of haemostasis (DFGSM2), research approach (PACES), conferences and training 3th cycle of medical studies (haematology), medical and scientific English (DCEM2 and 3), cell signalling mechanisms (M1 (Responsible of the M1), M2R, DIU).

Scientific activity and expertise
• Appointed member of Inserm Scientific Committee n°5 (2008-12) and scientific committees of: FRM (2012-16), La Ligue Nationale (2017-), INTS (2014-19), ARC-CR5 (president 2011-14), FDF (2006-12), lipidomic platform of Toulouse (president since 2011), SAB of Cochin Institute (2016-).
• Editorial board of Biochem J (2013-), Adv Biol Reg (2011-), Leukemia (2007-10). Advisory Editorial Board of J Thromb Haemost (2008-). Reviewer for Blood, Circulation Research, Haematological, JTH, TH, Nature Commun, BBA-lipids, etc. Evaluator of ANR, Welcome Trust, Italian Ministry of Research (PRIN), La Ligue Nationale, PHRC projects.
• Scientific committee « European Symposium on Hormones and Cell Regulation » (2005-), organizer of the 2009, 2014 and 2019 meetings. Co-organizer of the GEHT meeting (2002 and 2009), organizer of the 1st French-UK Platelet meeting (2009), founder and co-organizer of The Toulouse School of Lipids (since 2016).
• Mentor of 12 PhD thesis and 3 postdoctoral fellows, 8 of whom are independent investigators.
• Coordinator of research project (ANR project)
• Over 28 invited conferences abroad (FEBS, ETRO, ISTH, Keystone).

Awards
• CNRS Bronze medal 1998.
• Senior member of “Institut Universitaire de France” (2014-2019).
• Guillaumat Piel 2016 Price from « Fondation pour la Recherche Médicale ».
• FRM Team (2017-20 and 2022-2025).

Principal scientist

Marie-Pierre GRATACAP

INSERM researcher

Her current research focuses on the role of phosphoinositides on vascular integrity and the dialogue of the endothelium with platelets in different physiopathological contexts.

Career path
Master’s degree and PhD in Biology and Pharmacology of Hemostasis and Vessels at Paris VII (1996-1999). 2000-2003, Postdoc position at the Pharmacological Institute, University of Heidelberg, Germany). 2002-current INSERM researcher at the physiopathology centre of Toulouse and at the I2MC.

Main domains of expertise
Haemostasis and Thrombosis, Cardiovascular Physiology and Pathology, Actin cytoskeleton dynamics and phosphoinositide signaling.

Principal scientist

Karim HNIA

INSERM researcher

He develops research programs on how PI-metabolism regulate proteostasis in skeletal muscle and implications in muscle diseases such as myopathies and sarcopenia.

Career path
Master and PhD degrees in biochemistry and molecular biology at university of Montpellier (2002-2006), Postdoc position IGBMC, Strasbourg (2007-2011), junior researcher at the IGBMC (2012) and senior researcher at the I2MC (2016-current)..

Main domains of expertise
Phosphoinositides, proteostasis, trafficking, muscle disease and physiology.

Email:karim.hnia@inserm.fr

Tél/Phone:05 31 22 41 42

Twitter:@hnia_karim

Principal scientist

Sonia SEVERIN

INSERM researcher

Her current research focuses on the study of molecular and cellular mechanisms involving blood platelets and their precursors, megakaryocytes in different pathophysiological contexts.

Career path
Holds a Master’s degree in cell biology from Paul Sabatier Toulouse III (2002-06) and a MSC and PhD degrees in biology from l’Université Paul Sabatier Toulouse III University (2004-2007). After a postdoctoral experience in Birmingham (United-Kingdom) (2007-10), she joined B. Payrastre lab and got an Inserm permanent researcher position in 2013.

Main domains of expertise
Hemostasis, Thrombosis, Hematopoïesis, Cardiovasular and Metabolic Physiopathology, cell trafficking and signalling.

Email:sonia.severin@inserm.fr

Tél/Phone:05 31 22 41 43

Twitter:@severin_sonia

Principal scientist

Julien VIAUD

INSERM researcher

Julien Viaud is a cell biologist and a biochemist. His current research interests focus on the role of phosphoinositides and actin cytoskeleton dynamic in muscle cell differentiation and megakaryocyte interaction with extracellular matrices using genome engineered cell models.

Career path
Holds a Master’s degree in cell biology from Nantes University of and a MSC and PhD degrees in biology from Montpellier University (2007). After a postdoctoral experience in Philadelphia (2007-10), he joined B. Payrastre’s lab and got an Inserm permanent researcher position in 2016.

Main domains of expertise
Cell biology and biochemistry related to phosphoinositides signaling and actin cytoskeleton dynamic, genome editing.

Chercheure

Marie VANDROMME

INSERM researcher

Her current research focuses on the contribution of epigenetic mechanisms to the etiology of X-linked myotubular myopathy, a fatal pediatric disease due to mutations in the gene encoding the phosphoinositide 3-phosphatase MTM1.

Career path

Master’s degree and PhD in Biology&Health at University of Montpellier, France. HDR (University Paul Sabatier. 1995-1997 post-doctoral period at the National Institute for Medical Research, London (England). 1995-current INSERM researcher at the CRBM and IGH, Montpellier (1995-1999), at ENS Lyon (2000-2003), LBCMCP and CBI, Toulouse (2004-august 2021), and I2MC (September 2021-current).

Main domains of expertise

Proliferation and differentiation, skeletal muscle, Cancer, Myopathy, Chromatin, Histone modifications and gene expression, Transcription, Cell and molecular biology.

Clinician

Christophe COGNARD

Clinician

Thibault LHERMUSIER

Clinician

Agnès RIBES

Clinician

Pierre SIÉ

Clinician

Sophie VOISIN

Clinician, PhD student

Jean DARCOURT

Clinician, PhD student

Fanny VARDON-BOUNES

Research engineer INSERM

Gaëtan CHICANNE

Research engineer INSERM

He is a lipid biochemistry and molecular biology engineer. As part of the team’s research themes, his activities focus on development of new techniques to quantify phosphosinositides base on mass spectrometry and design news tools in molecular biology.

Career path
He holds a Master’s degree in biochemistry and a MSC in molecular biology from Paul Sabatier University – Toulouse. After a first experience at Bayer Cropscience in Lyon, he moved to B. Payrastre’s lab in 2003 and got an INSERM permanent engineer position in 2006.

Main domains of expertise
Lipid biochemistry (phosphoinositides quantification). Cellular and molecular biology (cloning, genome editing). Lentivirus production.

Engineer

Cédric GARCIA

Engineer

Jean-Marie Xuereb

Engineer University

He has expertise in hemostasis and Hematopoïesis. As part of the team’s research themes, his activities focus on exploring platelet homeostasis and function in related human diseases.

Career path
Master in Biotechnology and PHD in Biology in Toulouse in 1998, post-doctorate in R&D in private companies, recruited as IE2 university (2004-2008), attached to I2MC (2008-2012), finally IE2 then IEHC since 2018 in the team of B. Payrastre.

Main domains of expertise
Cellular and molecular biology, biochemistry, cytometry, imaging.

Engineer, Inserm

Anne MAZARS

Research Engineer

Career path
Anne received her PhD in Cancerology at University of Paris V followed by postdoctoral trainings in private companies and public laboratory. She joined our team in 2023 as an engineer to participate to the “newpi” project.

Main domain of expertise
Imaging, protein-protein interaction, cellular & molecular biology

Engineer

Marine MARSEILLAC

Engineer

Marine Marseillac is working on the characterization of new PI3Ks inhibitors in different in vitro cellular models.

Career path

Master’s degree in Biology-Health with a specialization in Physiopathology at Paul Sabatier University, Toulouse.

Main domains of expertise

Cellular biology, Molecular biology, Biochemistry.

PhD student

Laura BOUVET

PhD Student

Laura Bouvet joined the team in November 2022 as a PhD student to work on the role of PI3KC2β in endothelial dysfunction in a thrombo-inflammatory context.

Career path

Master ” Molecular and Cellular Biology pathway Microbiology, Infectious Diseases and Immunology ” at Grenoble Alpes University in 2022.

Main domains of expertise

Cellular and molecular biology, biochemistry of phosphoinositides

PhD student

Mathilde VAUCOURT

PhD Student

Mathilde Vaucourt studies the involvement of autophagy, more precisely mitophagy, during muscle differentiation using physiological and pathological cell models established by genome editing.

Career path

Research Master in Biology and Health at the Faculty of Science and Technology of Nancy (2022).

Main domains of expertise

Cell biology and biochemistry.

PhD student

Maxime BORRET

PhD Student

Maxime Borret works on the role of blood platelets in metabolic diseases in integrated models of pathologies in vivo and in cellular models in vitro.

Career path

Diploma in Agronomy from the Institut Agro de Rennes, formerly ENSAR (2020), M2R Molecular and Cellular Biology from the University of Rennes 1 (2020).

Main domains of expertise

Integrated physiopathology, molecular and cellular biology, histology

PhD student

Oportune KPOTOR

PhD Student

Oportune KPOTOR thesis project focuses on the involvement of MTM1, its products and substrates in beta 1 integrin trafficking and signaling in the context of X-linked myotubular myopathy ( XLMTM).

Career path

Master’s degree in Biology and Health – Pharmacological Innovation obtained in 2022 at the University of Paul Sabatier Toulouse III.

Main domains of expertise

Cell biology, molecular biology, signaling and cell trafficking.

PhD student

Nesrine HIFDI

PhD Student

Nesrine Hifdi studies the role of the ubiquitin ligase Fbxw7 in myogenesis and the link with phosphoinositide metabolism in the context of muscle differentiation using physiological and pathological cell models established by genome editing.

Career path

Master in Biology-Health (specialization in pathophysiology) at the University of Toulouse in 2021.

Main domains of expertise

Cellular and molecular biology, biochemistry of phosphoinositides

Phosphoinositide metabolism in cell fate and diseases

Coordinators : Karim HNIA, Julien VIAUD, Marie VANDROMME, Bernard PAYRASTRE

Our projects aim to investigate how phosphoinositides signaling regulate important cellular processes such as proteostasis, membrane and cytoskeleton dynamics as well as epigenetic. We develop bench to bedside strategies to model pathological contexts linked to defects in phosphoinositide metabolism particularly in megakaryocytes and muscle cells.

Platelet biology and its integrative function in organ physiology and pathology

 

Coordinators : Sonia SEVERIN, Marie-Pierre GRATACAP, Bernard PAYRASTRE 

Our projects aim to investigate platelet biology and how they dialogue with immune cells and with the endothelium to regulate organ functions (brain, heart, liver…) in normal and pathological contexts. We use cell biology, biochemistry and disease mouse models to investigate the role of platelets in cardiometabolic diseases and inflammation modulation, with translational outputs.

Platelet Signaling in clinical and translational research

 

Coordinators : Bernard PAYRASTRE, Agnès RIBES, Pierre SIE, Sophie VOISIN

Our projects aim to establish translational research in close interaction with the National Reference Center of Platelet Pathologies and clinical departments to investigate the molecular basis of acquired and genetic platelet pathologies. We use bench to bedside approaches to implement diagnostic methods, characterize new platelet pathologies and to improve patient care.

Selected publications

Impedance-based sensors discriminate among different types of blood thrombi with very high specificity and sensitivity.
Messina P, Garcia C, Rambeau J, Darcourt J, Balland R, Carreel B, Cottance M, Gusarova E, Lafaurie-Janvore J, Lebedev G, Bozsak F, Barakat AI, Payrastre B, Cognard C. J Neurointerv Surg. 2022. Pubmed

PIKfyve-Dependent Phosphoinositide Dynamics in Megakaryocyte/Platelet Granule Integrity and Platelet Functions. Caux M, Mansour R, Xuereb JM, Chicanne G, Viaud J, Vauclard A, Boal F, Payrastre B, Tronchère H, Severin S. Arterioscler Thromb Vasc Biol. 2022. Pubmed

Platelet activation and partial desensitization are associated with viral xenophagy in patients with severe COVID-19.
Garcia C, Au Duong J, Poëtte M, Ribes A, Payre B, Mémier V, Sié P, Minville V, Voisin S, Payrastre B, Vardon-Bounes F. Blood Adv. 2022. Pubmed

PI3KC2β inactivation stabilizes VE-cadherin junctions and preserves vascular integrity. 
Anquetyl T, Solinhac R, Jaffre A, Chicanne G, Viaud J, Darcourt J, Orset C, Göb E, Kleinschnitz C, Vanhaesebroeck B, Vivien D, Hnia K, Larrue V, Payrastre B, Gratacap MP
. EMBO Reports 2021. Pubmed

Adipocyte fatty acid transfer supports megakaryocyte maturation.
Valet C, Batut A, Vauclard A, Dortignac A, Bellio M, Payrastre B, Valet P, Severin S
. Cell Reports 2020. Pubmed

Catalytic dysregulation of SHP2 leading to Noonan syndromes impacts on platelet signaling and functions. 
Bellio M, Garcia C, Edouard T, Voisin S, Neel B, Cabou C, Valet P, Mori J, Mazharian A, Senis Y, Yart A, Payrastre B, Severin S. 
Blood 2019. Pubmed

The MTM1-UBQLN2-HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle.
Gavriilidis C, Laredj L, Solinhac R, Messaddeq N, Viaud J, Laporte J, Sumara I, Hnia K. 
Nature Cell Biology 2018. Pubmed

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