Team B. Payrastre

Lipidomic and signaling in platelet production, thrombosis and cell homeostasis (LIPSIPLAT)

Our laboratory studies phosphoinositides (PI) metabolism in health and disease. We use integrative and translational approaches to decipher PI-signalling and functions in various models including megakaryocyte, platelet and skeletal muscle. We also develop original translational research in the field of cardiovascular diseases in close interaction with clinicians.

TEAM MEMBERS

 
Tous / AllTeam leaderResearchersCliniciansStudentsTechnical staff
Team leader

gratacap

Principal scientist

Marie-Pierre GRATACAP

Principal scientist

Karim HNIA

Principal scientist

Sonia SEVERIN

Principal scientist

Julien VIAUD

Clinician

Christophe COGNARD

Clinician

Thibault LHERMUSIER

Clinician

Agnès RIBES

Clinician

Pierre SIÉ

Clinician

Sophie VOISIN

PhD student

Manuella CAUX

Clinician, PhD student

Jean DARCOURT

PhD student

Mélanie MANSAT

PhD Student

Mélanie PICOT

Clinician, PhD student

Fanny VARDON-BOUNES

Research engineer INSERM

Gaëtan CHICANNE

Engineer

Cédric GARCIA

Engineer

Alicia VAUCLARD

Engineer

Jean-Marie Xuereb

Team leader

gratacap

Professor of Haematology/Haemostasis (PU-PH), Medical Faculty Toulouse Purpan.

Responsible of the Functional Unit of “Cardiovascular Haemostasis”, Laboratory of Haematology, University Hospital of Toulouse.

Short description of positions held or career path
Diplomas: HDR, PhD thesis, Medical Pedagogy, Specialized electron microscopy.
2001-08: Director of Research at CNRS, group leader at Inserm U563, Toulouse.
2008: Professor of Haematology/Haemostasis (PU-PH), Medical Faculty Toulouse Purpan.

Teaching
Pathophysiology of haemostasis (DFGSM2), research approach (PACES), conferences and training 3th cycle of medical studies (haematology), medical and scientific English (DCEM2 and 3), cell signalling mechanisms (M1 (Responsible of the M1), M2R, DIU).

Scientific activity and expertise
• Appointed member of Inserm Scientific Committee n°5 (2008-12) and scientific committees of: FRM (2012-16), La Ligue Nationale (2017-), INTS (2014-19), ARC-CR5 (president 2011-14), FDF (2006-12), lipidomic platform of Toulouse (president since 2011), SAB of Cochin Institute (2016-).
• Editorial board of Biochem J (2013-), Adv Biol Reg (2011-), Leukemia (2007-10). Advisory Editorial Board of J Thromb Haemost (2008-). Reviewer for Blood, Circulation Research, Haematological, JTH, TH, Nature Commun, BBA-lipids, etc. Evaluator of ANR, Welcome Trust, Italian Ministry of Research (PRIN), La Ligue Nationale, PHRC projects.
• Scientific committee « European Symposium on Hormones and Cell Regulation » (2005-), organizer of the 2009, 2014 and 2019 meetings. Co-organizer of the GEHT meeting (2002 and 2009), organizer of the 1st French-UK Platelet meeting (2009), founder and co-organizer of The Toulouse School of Lipids (since 2016).
• Mentor of 12 PhD thesis and 3 postdoctoral fellows, 8 of whom are independent investigators.
• Coordinator of research project (ANR project)
• Over 28 invited conferences abroad (FEBS, ETRO, ISTH, Keystone).

Awards
• CNRS Bronze medal 1998.
• Senior member of “Institut Universitaire de France” (2014-2019).
• Guillaumat Piel 2016 Price from « Fondation pour la Recherche Médicale ».
• FRM Team (2017-20).

Principal scientist

Marie-Pierre GRATACAP

INSERM researcher

Her current research focuses on the role of phosphoinositides on vascular integrity and the dialogue of the endothelium with platelets in different physiopathological contexts.

Career path
Master’s degree and PhD in Biology and Pharmacology of Hemostasis and Vessels at Paris VII (1996-1999). 2000-2003, Postdoc position at the Pharmacological Institute, University of Heidelberg, Germany). 2002-current INSERM researcher at the physiopathology centre of Toulouse and at the I2MC.

Main domains of expertise
Haemostasis and Thrombosis, Cardiovascular Physiology and Pathology, Actin cytoskeleton dynamics and phosphoinositide signaling.

Principal scientist

Karim HNIA

INSERM researcher

He develops research programs on how PI-metabolism regulate proteostasis in skeletal muscle and implications in muscle diseases such as myopathies and sarcopenia.

Career path
Master and PhD degrees in biochemistry and molecular biology at university of Montpellier (2002-2006), Postdoc position IGBMC, Strasbourg (2007-2011), junior researcher at the IGBMC (2012) and senior researcher at the I2MC (2016-current)..

Main domains of expertise
Phosphoinositides, proteostasis, trafficking, muscle disease and physiology.

Email:karim.hnia@inserm.fr

Tél/Phone:05 31 22 41 42

Twitter:@hnia_karim

Principal scientist

Sonia SEVERIN

INSERM researcher

Her current research focuses on the study of molecular and cellular mechanisms involving blood platelets and their precursors, megakaryocytes in different pathophysiological contexts.

Career path
Holds a Master’s degree in cell biology from Paul Sabatier Toulouse III (2002-06) and a MSC and PhD degrees in biology from l’Université Paul Sabatier Toulouse III University (2004-2007). After a postdoctoral experience in Birmingham (United-Kingdom) (2007-10), she joined B. Payrastre lab and got an Inserm permanent researcher position in 2013.

Main domains of expertise
Hemostasis et Thrombosis, Hematopoïesis et Cardiovasular and Metabolic Physiopathology.

Email:sonia.severin@inserm.fr

Tél/Phone:05 31 22 41 43

Twitter:@severin_sonia

Principal scientist

Julien VIAUD

INSERM researcher

Julien Viaud is a cell biologist and a biochemist. His current research interests focus on the role of phosphoinositides and actin cytoskeleton dynamic in muscle cell differentiation and megakaryocyte interaction with extracellular matrices using genome engineered cell models.

Career path
Holds a Master’s degree in cell biology from Nantes University of and a MSC and PhD degrees in biology from Montpellier University (2007). After a postdoctoral experience in Philadelphia (2007-10), he joined B. Payrastre’s lab and got an Inserm permanent researcher position in 2016.

Main domains of expertise
Cell biology and biochemistry related to phosphoinositides signaling and actin cytoskeleton dynamic, genome editing.

Clinician

Christophe COGNARD

Clinician

Thibault LHERMUSIER

Clinician

Agnès RIBES

Clinician

Pierre SIÉ

Clinician

Sophie VOISIN

PhD student

Manuella CAUX

PhD Student (ANR)

She is working on the role of phosphoinositides in megakaryocytes and platelets, especially in intracellular trafficking.

Career path
Master’s degree in biotechnologies (specialization: Biomolecules, Micro-organisms and Bioproceeds) from Bretagne Sud University – Lorient (2018). PhD position (2018-current).

Main domains of expertise
Cell biology and microscopy, Megakaryocytes and platelets biology, Phosphoinositides signaling.

Clinician, PhD student

Jean DARCOURT

PhD student

Mélanie MANSAT

PhD Student (eRare)

She is working on the role of phosphoinositides in muscle cell differentiation using genome engineered cell models.

Career path
Master’s degree in cell biology (specialisation  in pharmacology) from Toulouse University (2017). PhD position 2018-current

Main domains of expertise
Cellular and molecular biology, lipid biochemistry.

PhD Student

Mélanie PICOT

PhD Student (AFM 3 years Fellow & Myotubular Trust 1 year fellow )

She is working on the role of phosphoinositides and their link to the mTORC1 signaling in skeletal muscle cell.

Career path
Master’s degree in cell biology (specialization in pharmacology) from Toulouse University (2018). PhD position (2018-current).

Main domains of expertise
Cell and molecular biology, lipid biochemistry.

Clinician, PhD student

Fanny VARDON-BOUNES

Research engineer INSERM

Gaëtan CHICANNE

Research engineer INSERM

He is a lipid biochemistry and molecular biology engineer. As part of the team’s research themes, his activities focus on development of new techniques to quantify phosphosinositides base on mass spectrometry and design news tools in molecular biology.

Career path
He holds a Master’s degree in biochemistry and a MSC in molecular biology from Paul Sabatier University – Toulouse. After a first experience at Bayer Cropscience in Lyon, he moved to B. Payrastre’s lab in 2003 and got an INSERM permanent engineer position in 2006.

Main domains of expertise
Lipid biochemistry (phosphoinositides quantification). Cellular and molecular biology (cloning, genome editing). Lentivirus production.

Engineer

Cédric GARCIA

Engineer

Alicia VAUCLARD

Engineer assistant

She has expertise in animal physiology, liver metabolism and blood platelet biology. She is working on the role of the blood platelet in metabolic diseases.

Career path
She holds a BTS diploma in BioAnalyses and Controls at the Lycée Saliège in Balma (Toulouse) and a professional License in Analalytic Biology and Experimentation at the I.U.T of Montpellier-Sète. She joined the I2MC (fixed-term contract since 2018).

Main domains of expertise
Animal experimentation, histology, cellular and molecular biology.

Engineer

Jean-Marie Xuereb

Engineer University

He has expertise in hemostasis and Hematopoïesis. As part of the team’s research themes, his activities focus on exploring platelet homeostasis and function in related human diseases.

Career path
Master in Biotechnology and PHD in Biology in Toulouse in 1998, post-doctorate in R&D in private companies, recruited as IE2 university (2004-2008), attached to I2MC (2008-2012), finally IE2 then IEHC since 2018 in the team of B. Payrastre.

Main domains of expertise
Cellular and molecular biology, biochemistry, cytometry, imaging.

Phosphoinositide metabolism in cell fate and diseases

Coordinators : Karim HNIA, Julien VIAUD, Marie VANDROMME, Bernard PAYRASTRE

Our projects aim to investigate how phosphoinositides signaling regulate important cellular processes such as proteostasis, membrane and cytoskeleton dynamics as well as epigenetic. We develop bench to bedside strategies to model pathological contexts linked to defects in phosphoinositide metabolism particularly in megakaryocytes and muscle cells.

Platelet biology and its integrative function in organ physiology and pathology

 

Coordinators : Sonia SEVERIN, Marie-Pierre GRATACAP, Bernard PAYRASTRE 

Our projects aim to investigate platelet biology and how they dialogue with immune cells and with the endothelium to regulate organ functions (brain, heart, liver…) in normal and pathological contexts. We use cell biology, biochemistry and disease mouse models to investigate the role of platelets in cardiometabolic diseases and inflammation modulation, with translational outputs.

Platelet Signaling in clinical and translational research

 

Coordinators : Bernard PAYRASTRE, Agnès RIBES, Pierre SIE, Sophie VOISIN

Our projects aim to establish translational research in close interaction with the National Reference Center of Platelet Pathologies and clinical departments to investigate the molecular basis of acquired and genetic platelet pathologies. We use bench to bedside approaches to implement diagnostic methods, characterize new platelet pathologies and to improve patient care.

Selected publications

PI3KC2β inactivation stabilizes VE-cadherin junctions and preserves vascular integrity. 
Anquetyl T, Solinhac R, Jaffre A, Chicanne G, Viaud J, Darcourt J, Orset C, Göb E, Kleinschnitz C, Vanhaesebroeck B, Vivien D, Hnia K, Larrue V, Payrastre B, Gratacap MP
. EMBO Reports 2021
. Pubmed

Adypocyte fatty acid transfer supports megakaryocyte maturation.
Valet C, Batut A, Vauclard A, Dortignac A, Bellio M, Payrastre B, Valet P, Severin S
. Cell Reports 2020.
Pubmed

Catalytic dysregulation of SHP2 leading to Noonan syndromes impacts on platelet signaling and functions. 
Bellio M, Garcia C, Edouard T, Voisin S, Neel B, Cabou C, Valet P, Mori J, Mazharian A, Senis Y, Yart A, Payrastre B, Severin S. 
Blood 2019. Pubmed

The MTM1-UBQLN2-HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle..
Gavriilidis C, Laredj L, Solinhac R, Messaddeq N, Viaud J, Laporte J, Sumara I, Hnia K. 
Nature Cell Biology 2018. Pubmed

A dual role of class III PI3K, Vp34, in platelet production and thrombus growth.
Valet C, Levade M, Chicanne G, Bilanges B, Cabou C, Viaud J, Gratacap M-P, Gaits-Iacovoni F,Vanhaesebroeck B, Payrastre B, Séverin S.
Blood 2017. Pubmed

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