Cytometry & Cell sorting Facility
Alexia ZAKAROFF-GIRARD / Elodie RIANT
firstname.lastname@example.org / 05.31.22.41.61
The I2MC cytometry Facility offers a wealth of experience and ressources in the use of flow cytometry for the analysis and sorting of cell populations harvested from solid tissues (heart, kidney, adipose tissue, gut...) for academic and private research investigators. It is partener of a group of similar facilities in the region, the Toulouse RIO Imaging (TRI) platform, IBiSa labelled and ISO9001 version 2015, NF X50-900 version 2016 certified.
We can advise you for your experiment design, train and assist you for acquisitions, analysis and interpertration of your data. We can also help you in the development of new research project in the form of collaboration.
In order to use the facility:
- Book an appointment to meet the staff to discuss the project.
- Read the contract of use of the platform TRI, the price list, the internal rules and the request for service form. These last two documents must be signed by every user and returned to the the facility at the time of the first meeting.
A compulsory training will be provided by the staff for first time users.
The analyzers are free access by reservation for users who have undergone mandatory training by staff. For others users, technical assistance is possible by appointment.
(405 nm, 488 nm, 561 nm et 633 nm)
- Cell sorter: Influx (BD Biosciences) – Optical configuration
The cell sorter makes it possible to isolate and recover one and more cell subspecies according to criteria of size, granulosity and fluorescence.
- The recovered cells can then be analyzed biochemically or returned to culture.
- 4 visible lasers (405 nm, 488 nm, 561 nm et 633 nm)
- HEPA filtered enclosure
- Isolate or enrich up to 6 different cell populations simultaneously, in tubes, culture plates or slides
- Cell cloning module (96-well plates)
- Cooling or heating samples module
- Microparticule detection module in FSC (<0.2 µm)
- Sort up to 50, 000 events per seconde
- Sorting is done by facility staff in the form of "service delivery" at the request of the research teams.