T. Mangeat will present a new versatile and Open Source superresolved microscopy methods based on dynamic speckle for a wide range of biological applications. He will demonstrate the ability to gaining confidence on living intracellular cell nanoscopy on many sensitive and complex biological situations. His system is expected to exhibit a resolution close to 100 nm transversally and 250 nm axially with a much greater ease of use. He will explain how to build the system and the generation of several speckle illumination patterns will be explained with simple binary phase SLM (a frame rate around 2ms). The low toxicity allows superresolved critical biological function study like mitosis, cell migration or subcellular force production during the morphogenesis process in thick tissue. Several Algorithm strategies will be explained, from simplest second central moment based to blind‐SIM and sparse based methods. These new methods combine structured illumination paradigm with fluctuation methods. The methods reduce the number of image need in bayesian or SOFI microscopy, and present a very robust compromise for living superresolved imaging in tissue.
Keywords : supperresolution, dynamic Speckle, inverse problem, versatile, live imaging, 3D imaging, Variance and Covariance, Tissu”
