team A. Bouloumié / R. Burcelin

DINAMIX: Adipose tissue, microbiota and cardiometabolic flexibility

Our research proposes an integrated and multidisciplinary approach to study the mechanisms of metabolic adaptation in the context of natural and accelerated ageing due to obesity and diabetes, based on the original concept of the determining role of the microbiota/adipose depots axis in the cardiometabolic flexibility.

L’Équipe

 
Tous / AllTeam leaderResearchersCliniciansPost Doctoral fellowsStudentsTechnical staff
Team leader

Anne BOULOUMIE-DIEHL

Co-team leader

Rémy BURCELIN

PhD, Inserm Researcher

Anaïs BRIOT

Researcher

Christian CARPENE

Researcher

Xavier COLLET

Researcher

Jean GALITSKY

Researcher

Christophe HEYMES

CLINICIAN

Jacques AMAR

CLINICIAN

Vincent BLASCO-BAQUE

Post Doctoral fellows

Nathalie BOULET

PhD Student

Alex AUPETIT

CLINICIEN - PhD Student

Matthieu MINTY

CLINICIAN - PhD Student

Charlotte THOMAS

Research Technician

Vincent AZALBERT

Assitant Ingineer INSERM

Pauline DECAUNES BESSEDE

Assitant Engineer

Albéric GERMAIN

Technical Researcher

Pascale LOUBIERES

Engineer

Joao PIRAQUIVE

Assitant Engineer

Jiuwen SUN

PHD, Operational manager of Cytometry Facility, Engineer

Alexia ZAKAROFF-GIRARD

Team leader

Anne BOULOUMIE-DIEHL

PhD, HDR, INSERM Research Director, Team Leader

Career path
PhD in pharmacology, director M. Lafontan, Université P Sabatier, Toulouse (1994); Post-doc, Cardiovascular Institute (R. Busse), Frankfurt, Germany (1994-1998); CRCN INSERM U598 (1998-2001); Kovalevskaja award, Frankfurt, Germany (2002-2006); AVENIR award, I2MR, Toulouse (2007-2011), Since 2012, INSERM team leader, I2MC.

Main domains of expertise  
Adipose tissue, progenitor, immune cells, endothelial cells, adipogenesis, inflammation, fibrosis, senescence, aging, obesity.

Co-team leader

Rémy BURCELIN

PhD, INSERM Research Director, Co-team Leader

Career path
PhD in endocrinology, Paris VI University (1992), Postdoctoral fellowship New York A. Einstein college or medicine (1993-1995), Assistant Professor University Lausanne (1995-2001), Professor University Toulouse (2001), Research Director INSERM at the Institute of Cardio and metabolic diseases.

Main domains of expertise  
Animal Physiology, Incretins, metagenomics, Energy Metabolism, Metabolic diseases and cardiovascular and hepatic complications.

PhD, Inserm Researcher

Anaïs BRIOT

PhD, Inserm Researcher

Career path
PhD in Molecular, Cellular and Integrated Pathophysiology, supervisor A Hovnanian (2009); Post-Doc Laboratory of L. Iruela-Arispe, UCLA, USA (2010-2015); Since 2016, CRCN INSERM Team Bouloumié, I2MC, Toulouse.

Main domains of expertise  
Vascular biology, cell biology, endothelial cells, adipose tissue, inflammation, aging, senescence.

Researcher

Christian CARPENE

PhD, INSERM Research 

Career path  
Christian Carpéné obtained his Doctorate of Science at the University of Toulouse in 1994. From 1979 to 1982, he worked as a pre- and post-doctoral fellow in the field of adrenergic pharmacology, and since 1983, he is a researcher at INSERM in Toulouse.

Main domains of expertise  
Biological effects of pharmacological, hormonal and nutritional compounds (polyphenols, dietary amines) on the metabolic and endocrine functions of adipocytes.

Researcher

Xavier COLLET

PhD, HDR, Research Director INSERM 

Career path
PhD in Biochemistry (1988), Post-Doc in the laboratory of C. Fielding (1988-1991), Sabbatical stay: Columbia University, Department of Medicine, New-York, (A. Tall).  Since 2021, DR INSERM in Bouloumié team, I2MC, Toulouse. Director of CREFRE (July 2020), Director of SFR-BMT (since 2011), Co-director of Génotoul (since 2011).

Main domains of expertise  
Lipoprotein metabolism, lipids, cholesterol, intestinal absorption of lipids, microbiota.

Researcher

Jean GALITSKY

PhD, INSERM Research 

Career path
PhD in molecular pharmacology, director M Lafontan, Université P Sabatier, Toulouse (1989); Post-doc, Karolinska Institute, laboratory P.Arner, Stockholm, Sweden (1989); Since 1990, INSERM Researcher; Since 2007, AVENIR/Bouloumié Team, I2MC, Toulouse, France.

Main domains of expertise  
Adipose tissue, progenitor, adipogenesis, fibrosis, obesity, microdialysis.

Researcher

Christophe HEYMES

PhD, Senior Researcher INSERM 

Career path
PhD in Membrane Physiology and Pharmacology, PhD supervisor B. Swynghedauw (1997); Post-Doc Laboratory of Pr. Ajay M. Shah, Kings College of London, UK (1998-2001); Recruited INSERM (CR1) in 2001. Since 2008, DR2 INSERM.

Main domains of expertise  
Cardiac physiology, cell biology, aging, senescence, heart failure.

CLINICIAN

Jacques AMAR

MD medical doctor ; University professor and and hospital practitioner

Career path
Habilitation to conduct Research (2000); University Professor of Therapeutics ( 2001) ; Exceptional class (2015).

Main area of expertise
Blood and tissue Microbiota, Hypertension, Diabetes.

CLINICIAN

Vincent BLASCO-BAQUE

DMD, PhD, Associated professor and hospital practitioner

Career path
DMD in Dental Surgery (2009); PhD in Biology, Health and Biotechnology, specialty Physiopathology, supervisor R. Burcelin (2013); DIU in Endodontics (2014); MCU-PH UFR Santé de Toulouse (2016); HDR (2019).

Main area of expertise
Dentistry, oral microbiota, metabolic disease, periodontitis, Porphyromonas gingivalis

Post Doctoral fellows

Nathalie BOULET

PhD, Post-doctoral fellow

Career path
PhD in Physiopathology, PhD supervisors J Galitzky/A Bouloumié (2015); Post-Doc Laboratory of J Nedergaard and B Cannon, Stockholm, Sweden (2015-2018); Since 2018, post-doc, Equipe Bouloumié, I2MC, Toulouse.

Main domains of expertise  
Progenitor cells, white and brown adipose tissues, adipogenesis, myofibrogenesis, obesity, mitochondrial activity.

PhD Student

Alex AUPETIT

PhD Student

Career path
Master in Biology and Health, specialty Physiology, Physiopathology and Pharmacology, University of Poitiers (2020); Since October 2020, PhD student under the supervision of Anaïs Briot “Metabolic function of the adipose tissue microvasculature: a target of obesity and aging”, Team Bouloumié, I2MC, Paul Sabatier University, Toulouse, INSERM/région Occitanie funding.

Main area of expertise
Vascular physiology, Adipose tissue, Aging.

CLINICIEN - PhD Student

Matthieu MINTY

DMD, University Hospital Assistant, Faculty of Dental Surgery, PhD Student

Career path
DMD, Faculty of Dental Surgery of Paul Sabatier (2017) ; Master in Biology and Health specialty Physiopathology of Infections, University of Paul Sabatier Toulouse (2019) ; Since Sept 2020, PhD student under the direction of V. Blasco “the oral microbiota: a major actor of our general health”, Tema Burcelin, I2MC, University Paul Sabatier, Toulouse.

Main area of expertise
Dental surgery, oral microbiota, metabolic diseases, Porphyromonas ginigvalis.

CLINICIAN - PhD Student

Charlotte THOMAS

DDS, University Hospital Assistant, Faculty of Dental Surgery, PhD Student

Career path
Doctor of Dental Surgery (DDS, 2018), Faculty of Dental Surgery of Toulouse; Residency 2015-2018: Specialization in Oral Medicine, Faculty of Dental Surgery of Toulouse; 2019 Specialization in Periodontology – University Diploma, University of Paul Sabatier Toulouse; 2019 Master Biology Health: Physiopathology from molecular to medical, University of Paul Sabatier Toulouse;
Since Sept 2019, PhD student under the supervision of Dr Vincent Blasco-Baque and Dr Sara Laurencin-Dalicieux: “Oral microbiota and obesity: from a dysbiotic oral microbiota signature in humans to its causality in mice”, Paul Sabatier University, Toulouse

Main area of expertise
Dental surgery, oral microbiota, periodontitis, obesity.

Research Technician

Vincent AZALBERT

Research Technician INSERM

Career path
BTS ABM Biology and Medical Analysis (2007-2009), recruited at INSERM since 2009.

Main domains of expertise  
Molecular Biology (DNA-RNA extraction, PCR, RT-qPCR), Cell Biology (adipose tissue progenitor cell culture), Immunology (immune cell labelling, FACS, t-SNE), Biochemistry (ELISA assays)

Assitant Ingineer INSERM

Pauline DECAUNES BESSEDE

Assistant Engineer INSERM

Career path
Licence professionnelle de Biotechnologies, Université Versailles- St Quentin en Yvelines, (2004); Assistant Engineer, INRA Jouy-en-Josas (2004-2006), INSERM Equipe AVENIR/Bouloumié, I2MR, Toulouse (2006-2009); TCN INSERM Equipe Bouloumié, I2MC, Toulouse (2010-2019); Since 2019, AI INSERM Team Bouloumié, I2MC.

Main area of expertise
Isolation and culture of human and mouse vascular and stromal cells by flow cytometry and/or magnetic beads; Approaches: Imaging, transcriptional, protein and lipid analyses.

Assitant Engineer

Albéric GERMAIN

Engineering assistant

Career path
Master in Biology, Ecology, Evolution, specialization in Ecological System Modeling, University of Toulouse III (2015-2017) ; Research Assistant, University of Toulouse III, CRCA (2018) ; since 2020 Engineering Assistant, I2MC, Toulouse

Main domains of expertise
Data analysis, Biostatistics, omics data.

Technical Researcher

Pascale LOUBIERES

Technical Researcher and Training Assistant, Toulouse Faculty of Dental Surgery

Career path
BTS ANABIOTEC ITSA La Raque Lasbordes (1994); since 1995 Technical Researcher and Training Assistant, Paul Sabatier University, and since 2010, Burcelin Team, I2MC, Toulouse.

Main area of expertise
General and oral microbiology, animal experimentation, molecular biology.

Engineer

Joao PIRAQUIVE

PhD, Engineer

Career path
2018 PhD in biomedical imaging/preclinical MRI, Paris Diderot university; 2019-2021 : Post-Doc University of California San Francisco (UCSF, USA) ; MR research scientist CREFRE-ENI et I2MC since June 2021.

Main area of expertise
MRI, MRI contrast agents, spectroscopy, physics, inflammation.

Assitant Engineer

Jiuwen SUN

Assistant Engineer INSERM

Career path
Master of SCIENCES AND TECHNOLOGY OF AGRICULTURE FOOD AND THE ENVIRONMENT INTEGRATIVE BIOLOGY (OMICS),  University Paris Est Créteil Val Sur Marne  (2019-2021); Since August 2021 Engineering Assistant, I2MC, Toulouse

Main area of expertise
Data analysis, Biostatistics, omics data.

PHD, Operational manager of Cytometry Facility, Engineer

Alexia ZAKAROFF-GIRARD

PhD, Operational manager, Engineer

Alexia Zakaroff-Girard received her PhD from Claude Bernard University (Lyon) in 1997 where she studied the role of biologically active phospholipids in immune response. She completed post-doctoral training at I2MC in adipose tissue physiology. Since 10 years, she is heading the Cytometry and Cell Sorting Facility at I2MC, offering a wealth of experience and resources in the use of flow cytometry for the analyses and sorting of cell populations harvested from solid tissues for academic and private research investigators.

Main domains of expertise:
Flow cytometry, Cell sorting, Adipose tissue physiology, Cellular Biology

Microbial dysbiosis, adipose depot heterogeneity and cardiometabolic dysfunction

 

Principal investigators : Vincent Blasco-Baque, Xavier Collet, Rémy Burcelin

Our objective is to create and to analyze large-scale databases (metagenomic, transcriptomic, metabolomic) of adipose depots as well as tissue and oral microbiomes from human and mouse cohorts (including axenic mouse lines colonized by human microbiota) in context of obesity and ageing taking into account sex. Links with clinical/biological data are analyzed by machine learning strategies (development of new algorithms) in order to extract information that classifies and/or predicts cardiometabolic phenotypes and to propose new molecular relationships between tissue bacteria and adipose depot cells.

Metabolic function of microvascular endothelium: Heterogeneity, nutrient sensing and handling

 

Principal investigators : Anaïs Briot, Anne Bouloumié

Our work on native and primary microvascular endothelial cells from subcutaneous and visceral human and mouse adipose depots aims at 1) identifying the cellular and molecular mechanisms involved in nutrient sensing and transport, 2) defining the inter- and intra-fat depot phenotypic and functional heterogeneity of endothelial cells and, 3) assessing the impact of obesity and aging on adipose depots endothelial cells metabolic function in human and mice.

From progenitor cells to mature adipocytes: Heterogeneity, differentiation and function

 

Principal investigators : Jean Galitzky, Christian Carpéné, Anne Bouloumié

Our objective is to define the role of bacteria and their derivatives in the intrinsic and extrinsic molecular mechanisms governing the heterogeneity of 1) progenitor cells in terms of their adipogenic (white, beige and brown) and myofibrogenic fate and their intra-lobular and inter-depot location and 2) adipocyte function.

Dynamics and adaptation of the microbiota/adipose depot axis:

Metabolic flexibility and fasting

 

Principal investigator : Anaïs Briot

Using distinct fasting protocols, our objectives are 1) to identify dynamic combinations of markers (metabolomic, transcriptomic and metagenomic) of metabolic inflexibility, 2) to assess the beneficial and deleterious consequences on the phenotype and functionality of adipose depot cells, and 3) to identify cellular and molecular mechanisms that selectively mimic the beneficial effects of intermittent fasting according to sex, age and metabolic health.

Dynamics and adaptation of the microbiota/adipose depot axis:

Metabolic flexibility and pre/probiotics

 

Principal investigator : Christophe Heymes

Using nutritional approaches (based on the use of prebiotics), the objectives during the development of obesity/diabetes are 1) to study intestinal and tissue dysbiosis (adipose tissue and heart); 2) to study the effects of Polydextrose (prebiotic) on dysbiosis and the impact on cardiac dysfunction; 3) to identify new probiotics and bacterial metabolites responsible for the observed effects; 4) to identify molecular mechanisms (peptides, proteins secreted by the intestine and/or the adipose tissue) mediating in part the observed effects.

RECENT PUBLICATIONS

Obesity of mice lacking VAP-1/SSAO by Aoc3 gene deletion is reproduced in mice expressing a mutated vascular adhesion protein-1 (VAP-1) devoid of amine oxidase activity, Jargaud V, Bour S, Tercé F, Collet X, Valet P, Bouloumié A, Guillemot JC, Mauriège P, Jalkanen S, Stolen C, Salmi M, Smith DJ, Carpéné C. J Physiol Biochem. 2021. Pubmed

Detection of representative variables in complex systems wtih interpretable rules using Core-Clusters. Champion, C., A. C. Brunet, R. Burcelin, J. M. loubes and l. Risser. Algorithms 2021. Pubmed

Molecular phenomics and metagenomics of hepatic steatosis in non-diabetic obese women. Hoyles, L., J. M. Fernandez-Real, M. Federici, M. Serino, J. Abbott, J. Charpentier, C. Heymes, J. L. Luque, E. Anthony, R. H. Barton, J. Chilloux, A. Myridakis, L. Martinez-Gili, J. M. Moreno-Navarrete, F. Benhamed, V. Azalbert, V. Blasco-Baque, J. Puig, G. Xifra, W. Ricart, C. Tomlinson, M. Woodbridge, M. Cardellini, F. Davato, I. Cardolini, O. Porzio, P. Gentileschi, F. Lopez, F. Foufelle, S. A. Butcher, E. Holmes, J. K. Nicholson, C. Postic, R. Burcelin and M. E. Dumas. Nat Med. 2018. Pubmed

Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells. Estève D, Boulet N, Belles C, Zakaroff-Girard A, Decaunes P, Briot A, Veeranagouda Y, Didier M, Remaury A, Guillemot JC, Ledoux S, Dani C, Bouloumié A, Galitzky J. Nat Commun. 2019. Pubmed

Senescence Alters PPARγ (Peroxisome Proliferator-Activated Receptor Gamma)-Dependent Fatty Acid Handling in Human Adipose Tissue Microvascular Endothelial Cells and Favors Inflammation. Briot A, Decaunes P, Volat F, Belles C, Coupaye M, Ledoux S, Bouloumié A. Arterioscler Thromb Vasc Biol. 2018Pubmed

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