Facility Manager : Frédérique Gaits
- Zeiss LSM510 confocal microscope
This microscope is equipped with 3 lasers (argon: 458, 488,514 nm; 2 helium-neon lasers: 543 and 633 nm) on an inverted body. Lenses are: 10x and a 25x for global observation; a 40x and a 63x for higher magnification. There is a possibility of thermoregulation to perform live cell imaging. The FCS confocor module allows monitoring of protein-protein interactions. It is piloted by the Zeiss LSM software.
- Confocal Microscop Zeiss LSM780
The Oberserver Z1 inverted microscope is equipped with 3 sources of laser illumination: Diode 405 nm, Argon (458, 488, 514nm), DPSS : 561nm and HeNe 633 nm. A highly sensitive GaAsp detector with a quantum yield of about 45% in a wide range of wavelength and an online spectral detection over 34 channels in lambda mode are available on this machine. This microscope is equipped with a FCS module (Fluorescence Correlation Spectrometry) and is ready to use F-techniques (FRET, FRAP and so on). The microscope is also fully equiped with a system of environmental control (temperature and CO2 monitoring) to image live cells over long period of time. All classical cell culture supports can be installed on the microscope (35 mm Ibidi chambers, Labtek, etc….).
It is piloted by the ZEN 2012 black edition, with advanced applications (3D reconstructions, projections, quantification, colocalization...). The spectral detector allows imaging of up to 8 fluorophores in parallel on the same sample, changing parameters in x/y/z, as well as using the mosaic function at the same time. Up to 5D can be integrated in real time.
- Station of video-microscopy Wide Fiel Zeiss Cell-Observer
The inverted Zeiss Cell Observer Z1 video-microscope is equipped with a chamber for thermo and CO2 regulation for optimal live cell imaging.
- Two Hamamatsu cameras, an ORCA R² and an EM-CCD allow choosing between image quality (sensitivity) or increased speed respectively, according to the experimental design and the biological parameter that is being followed. This system is adapted to a wide variety of experiments, it offers many different lenses and excitation/emission filters.
- Two illumination devices:
1. The fast COLIBRI LED system allows convenient change of excitation light. 4 wavelength are available: Green (GFP), Red (CY3), Blue (DAPI), far Red (CY5)
2. Other wavelength can be choosen by working with the HXP lamp and sets of filters on fast weels. This machine is spoecifically equipped for CFP/YFP FRET monitoring.
This microscope is actually used for microfluidic experiments with various set ups (Cellix, Ibidi, Bioflux).
This configuration allows investigating live cells at very fast rate (a few seconds for in vitro thrombi formation in microfluidic) and also over long periods of time (the system is stable up to 8 days). The system is adapted to observation on various culture supports (plastic or glass bottom, multiwells plates, Labtek etc)It is piloted by the ZEN 2012 blue edition, with optimized acquisition and processing modules. Multi positions, z stacks, mosaics acquisition are possible, the system is stabilized by the « definite focus » module for long time imaging.
- Super-resolution Microscopy with the Zeiss ELYRA PS. 1 station
This machine allows parallel investigation of super-resolved subcellular structure applying different techniques:
- SR-SIM technology: the « structured illumination microscopy » allows to reach a resolution of about 100 nm, in three colors (using classical fluorophores or fluorescent proteins, compatible with live cell imaging) in 3D.
- PALM/STORM technology: the « photo-activated localization microscopy » allows to lower the resolution to a few tenth of nm using photo-activatable fluorophores or fluorescent proteins. The « stochastic optical reconstruction microscopy » allows to go down to the single molecule.
This microscope is equipped with a 3D module, and is able to image the same sample with the 3 different techniques if adequate fluorophores are used. It is also equipped with a TIRF module to assess events at/close to the plasma membrane.