Direct access to content

20 août

You are here : Home > Research > Research teams

Team 2

FUNCTIONAL GENOMIC OF METABOLIC DISEASES


Our research focuses on the understanding of the molecular mechanisms controlling blood glucose homeostasis. We are now focussing on intestinal risk factors such as intestinal microbiota and lipids for the control of the gut to brain GLP-1 dependent axis during diabetes and dyslipidemia.
We have set up animal models and in vivo methods of functional genomic required for the assessment of major physiological functions involved in the regulation of glucose homeostasis i.e. insulin sensitivity, hepatic glucose production, individual tissue glucose utilization, pancreatic and intestinal secretion, arterial blood flow, brain infusions, intestinal lipid absorption...
Using this in vivo physiological approach on transgenic mice we were hence the first to describe some of the molecular component of the enteric nervous system for the detection of glucose: GLUT-1, and the GLP-1 receptor. We then showed the importance of GLP-1 on the gut-brain axis for the control of glucose and vascular homeostasis. We further showed that this axis is impaired during diabetes. Consequently, we proposed that metabolic inflammation could be responsible for the altered gut-brain axis. We showed that the Lipopolysaccharide, a component of the Gram negative bacterial wall is a triggering factor of a CD14 dependent system for the onset of metabolic diseases. We suggest that LPS could impair the gut-brain axis by a mechanism requiring inflammation. We are now studying the relationship between intestinal microbiota, the immune response and the occurence of diabetes and dyslipidemia as risk factor for the gut to brain GLP-1 dependent axis and the control glucose metabolism.

Expected health effects

Demonstrate 1) that intestinal microbiota and lipids are riskfactors for the control of diabetes and dyslipidemia;

2) that these risk factors regulate the enteric GLP-1 dependent gut to brain
neural axis;

3) that the innate and acquired immune system is a major regulator of the host to bacteria for the control of diabetes and dyslipidemia

Cooperation and Partnerships

Our partnerships are international :

Canada, Toronto , D. Drucker : Role of the inhibitors of DPPIV for the control of the action of GLP-1.

Switzerland, Lausanne: B Thorens, role of the GLUT2 glucose transporter on the control of brain GLP-1. Lausanne: Urs Scherrer: action of brain insulin, nitric oxide, and GLP1 on the control of arterial femoral blood flow.

Belgique:Bruxelles: role dietary fibers on inflammatory reaction during diabetes.

Italie, Rome: role ofTImp3 on the control of metabolic diseases.

Numerous collaborations with local reasearch teams of the institute. Numerous industrial partners, Physiogenex, Nestlé, DANISCO, MSD...

Managers

Rémy BURCELIN






Xavier COLLET

Curriculum Vitae
Main publications






The team

AZALBERT Vincent (TN)
BLASCO Vincent (Doc)
BURCELIN Rémy (DR1)
CAMPISTRON Gérard (PU)
COLLET Xavier (DR2)
COLOM André (AI)
GARIDOU Lucile (Post doc)
GARRET Céline (AI)
KLOPP Pascale (AI)
LOUBIERES Pascale (TN)
PAYROS Gaëlle (AI)
POMIE Celine (Post doc)
REICHARDT François (Post doc)
SERINO Matteo (Post doc)
TERCE François (CR1)
WAGET Aurélie (AI)


Picture legend














Role of intestinal microbiota

Date of update November 26, 2013


Version Française


Contact

Rémy BURCELIN
Tél : 05 61 32 56 14
Fax : 05 61 32 56 21
e-mail : remy.burcelin@inserm.fr
Address :
Inserm/UPS  UMR 1048 - I2MC, Equipe 2
1 avenue Jean Poulhès
BP 84225
31432 Toulouse Cedex 4

Keywords


Diabetes
Fonctionnal exploration
Obesity
Metabolic diseases
Insulin resistance
Autonomous nervous system
Vascular bloor flow
Inflammation
Intestinal microbiota
GLP-1 signaling
Intestinal absorption
Animal models

Biological ressources


Liver gene expression, metagenomic in feces (Patients NAFLD)
Diabetes and obesity animal models
CACO2 cells
Intestinal  lipid transport

Methodologies used


- State of the art techniques unique in France to study in vivo in the awake free moving mouse glucose
(tracers) and vascular homeostasis (ultrasonic probe) simultaneously with brain infusate to respect the
integrity of the physiological systems and to address molecular issues.
- Vagus nerve recording.
- Blood hormone biochemistry.
- Metagenomique, bioinformatique, metafactors secretome (secreted bacterial product library)
- Accurate immunological phenotyping (FACS, lymphocyte transfer, confocal analyses...)
- Lipidology, enteric cell culture, molecular fluorescent tools for the analysis of lipid handing

Picture library

Unité 1048 ou I2MC -Institut des Maladies Métaboliques et Cardiovasculaires, 1 avenue Jean Poulhès - BP 84225, 31432 Toulouse Cedex 4
Inserm Université de Toulouse Université Toulouse III - Paul Sabatier